anti-Human, Mouse IkB-alpha Monoclonal Antibody | anti-IkB-alpha antibody

Anti-IkB-alpha (N-term) Mouse mAb

Cat. Num. AAA475019

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: IkB-alpha; Monoclonal Antibody; Anti-IkB-alpha (N-term) Mouse mAb; I kappa B alpha; I (Kappa)B (alpha); I-kappa-B-alpha; IkappaBalpha; IKBA; IKBA_HUMAN; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; anti-IkB-alpha antibody

• Host: Mouse; Source: Human
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: 2E10-B11-F6
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-IkB-alpha antibody: Western Blot (WB)
• Application Notes: WB: 1:1000; Other: 1:500
• Immunogen: Purified recombinant human IkB-alpha (N-terminus) protein fragments expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of IkB-alpha (N-terminus) and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the NF-kappa-B inhibitor family. Contains 5 ANK repeats.
• Subunit Structure: Interacts with RELA; the interaction requires the nuclear import signal. Interacts with NKIRAS1 and NKIRAS2. Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with HBV protein X. Interacts with RWDD3; the interaction enhances sumoylation. Interacts (when phosphorylated at the 2 serine residues in the destruction motif D-S-G-X (2, 3, 4)-S) with BTRC. Associates with the SCF (BTRC) complex, composed of SKP1, CUL1 and BTRC; the association is mediated via interaction with BTRC. Part of a SCF (BTRC)-like complex lacking CUL1, which is associated with RELA; RELA interacts directly with NFKBIA. Interacts with PRMT2.
• Subcellular Location: Cytoplasm. Nucleus

Western Blot (WB)

(Western blot detection of IkB-alpha (N-terminus) in Jurkat and Hela cell lysates using IkB-alpha (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.)

Western Blot (WB)

(Western blot detection of IkB-alpha (N-terminus) in 3T3 cell lysate using IkB-alpha (N-terminus) mouse mAb (1:500 diluted).Predicted band size: 36KDa.Observed band size: 36KDa.)

Related Product Information for anti-IkB-alpha antibody

Entrez Summary: This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NM_020529
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 36
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: NFKB inhibitor alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.