Rabbit anti-Human, Mouse NELFE Polyclonal Antibody | anti-NELFE antibody

NELFE Polyclonal Antibody

Cat. Num. AAA2555804

• Gene Names: NELFE; RD; RDP; RDBP; D6S45; NELF-E
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: NELFE; Polyclonal Antibody; NELFE Polyclonal Antibody; Major histocompatibility complex gene RD; Negative elongation factor E; Negative elongation factor polypeptide E; NELF e; NELF-E; Nelfe; Nuclear protein; RD; RD protein; RD RNA binding protein; RDP; RNA-binding protein RD; anti-NELFE antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 387

• Applicable Applications for anti-NELFE antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human NELFE
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NELFE antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using NELFE antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse Intestine using NELFE antibody.)

Related Product Information for anti-NELFE antibody

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.

Product Categories/Family for anti-NELFE antibody

Cancer; Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 14670268
• NCBI GeneID: 7936
• NCBI Accession #: NP_002895.3
• NCBI GenBank Nucleotide #: NM_002904.6
• UniProt Accession #: P18615; Q5JP74; Q5JP75; Q96F56; Q9NPK2; A2BE08; B4DUN1; B4DYX9
• Molecular Weight: Calculated: 40kDa; 43kDa; Observed: 43kDa
• NCBI Official Full Name: negative elongation factor E
• NCBI Official Synonym Full Names: negative elongation factor complex member E
• NCBI Official Symbol: NELFE
• NCBI Official Synonym Symbols: RD; RDP; RDBP; D6S45; NELF-E
• NCBI Protein Information: negative elongation factor E
• UniProt Protein Name: Negative elongation factor E
• UniProt Gene Name: NELFE
• UniProt Synonym Gene Names: RD; RDBP; NELF-E

NCBI Description

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.