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Gene Name
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Host
Application
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2246 results for " Loading Control Antibodies" - showing 1-50
Immunofluorescence (IF)
(Firure 1. Immunofluorescent Validation of MBS150780 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021)Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, MBS150780. (k, n) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k); pan-macrophage marker CD68 (magenta) (n) and DAPI (blue).)
COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA150780)
Full Name
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody
Reactivity
Virus
Applications
IF, IHC, WB
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of PD-L1 in HeLa CellsLoading: 15ug of lysates per lane. Antibodies: MBS151123 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
PDL-1, Polyclonal Antibody (Cat# AAA151123)
Full Name
PDL-1 Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC-P, IF, FC
Purity
PD-L1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Published customer image:Confocal Microscopy of Mitotic Cells Stained for a-Tubulin and the Nucleolar Protein BN46/51. Five examples of progressively later stages of each mitotic stage are presented. Each image is a Z-axis projection of 0.25 um optical sections through an individual cell. The cell periphery is outlined in white. A. Interphase; B. Prophase; C. Metaphase; D. Anaphase; E. Telophase. F. Selected nuclei of, from left to right, Prophase, Metaphase, Anaphase, and Telophase. Bar = 10 um. Red, BN 46/51; Green, a-tubulin.From: Walsh CJ (2012) The Structure of the Mitotic Spindle and Nucleolus during Mitosis in the Amebo-Flagellate Naegleria. PLoS ONE 7(4): e34763.)
TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA213160)
Full Name
RAT ANTI TUBULIN ALPHA
Applications
EIA, IF, RIA, WB
Pricing
$645/0.5 mg | $2,755/5x0.5 mg
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA214794)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC, IF
Pricing
$195/0.025 mg | $730/5x0.025 mg
Immunohistochemistry (IHC)
(MBS9601318 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ZO 1, Polyclonal Antibody (Cat# AAA9601318)
Full Name
ZO 1 Antibody
Gene Names
TJP1; ZO-1
Reactivity
Human, Mouse, Rat, Pig, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TACE 1131 (0.5 μg/mL), TACE 22-001 (2 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
TACE, Polyclonal Antibody (Cat# AAA150091)
Full Name
TACE Antibody
Gene Names
ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IF, FC/FACS
Purity
TACE Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of BACELoading: 15 ug of lysates per lane. Antibodies: BACE (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A-C: human brain tis)
BACE, Polyclonal Antibody (Cat# AAA150291)
Full Name
BACE Antibody
Gene Names
BACE1; ASP2; BACE; HSPC104
Reactivity
Human, Mouse
Applications
EIA, ICC, IF, IHC-P, WB
Purity
BACE Antibody is Ion exchange chromatography purified.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of CXCR4 in HeLa CellsLoading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP )
CXCR4, Polyclonal Antibody (Cat# AAA151360)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IP, IF, IHC-p, FC
Purity
CXCR4 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA212026)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS, IF
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of MyD88 in HeLa (A) and Jurket (B) CellsLoading: 15 μg of lysates per lane.Antibodies: 2125 (1 μg/mL) 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
MYD88, Polyclonal Antibody (Cat# AAA150754)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of MyD88 in human cell linesLoading: 15 μg of lysates per lane.Antibodies: 2127 (2 μg/mL) 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Predicted band size: 35 kDa)
MYD88, Polyclonal Antibody (Cat# AAA151266)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation of PUMA in K562 and 3T3/NIH CellsLoading: 15 μg of lysates per lane.Antibodies: 3041 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
PUMA, Polyclonal Antibody (Cat# AAA151208)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human, Mouse
Applications
EIA, WB, ICC, IF
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Western Blot of Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 ug per lane. Protein staining shown below each blot depicts equal protein loading. anti-Collagen I antibody at 0.22 ug/10 ml for overnight at 4 deg C. horseradish peroxidase-conjugated rabbit secondary antibody at 1 ug/10 ml for overnight at 4 deg C. Block: TBS with 5% Non-fat milk. Size: 138.9 kDa for Collagen I.)
Collagen Type I, Polyclonal Antibody (Cat# AAA534202)
Full Name
Collagen Type I antibody (biotin)
Gene Names
COL1A1; OI1; OI2; OI3; OI4; EDSC
Reactivity
Reacts with Human, Bovine, Rat, and Mouse. Less than 0.1% cross-reactivity with Type II, III, IV, V, or VI Collagens.
Applications
EIA, IHC, IP, WB
Purity
Collagen type I antibody (biotin) was purified by affinity chromatography.
Pricing
$910/0.1 mg | $4,090/5x0.1 mg
Testing Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 647 (MBS212342A647))
CD81, Monoclonal Antibody (Cat# AAA210783)
Full Name
HAMSTER ANTI MOUSE CD81:FITC
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Applications
FC
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Testing Data
(Figure 1 Overexpression Validation of PD-L1 in 293 CellsLoading: 15 μg of lysates per lane.Antibodies: (A, 0.25 μg/mL; B, 0.5 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.)
PDL1, Monoclonal Antibody (Cat# AAA154572)
Full Name
PDL1 Antibody [6H10]
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
EIA, WB, IHC, ICC, IF
Purity
Protein A purified
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Testing Data
(Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane. Antibodies: ACE2, MBS150723 (2 ug/mL), ACE2, MBS150187 (2 ug/mL), ACE2, MBS151315 (2 ug/mL) and beta-actin MBS150383 (1 ug))
ACE2, Polyclonal Antibody (Cat# AAA150723)
Full Name
ACE2 Antibody
Gene Names
ACE2; ACEH
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
ACE2 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Loading: 15 ?g of lysates per lane. Antibodies: RIP3 (MBS150424), (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
RIP3, Polyclonal Antibody (Cat# AAA150424)
Full Name
RIP3 Antibody
Gene Names
Ripk3; Rip3; AW107945; 2610528K09Rik
Reactivity
Human, Mouse, Rat
Applications
ELISA, IF, IHC-P, IP, WB
Purity
RIP3 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: DR4 1139 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: HepG2 cell lysateLane B: Jurkat cell lysate)
DR4, Polyclonal Antibody (Cat# AAA151363)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
EIA, WB, ICC
Purity
DR4 Antibody is Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
alpha-Tubulin mAb, Antibody (Cat# AAA355016)
Full Name
alpha-Tubulin antibody
Reactivity
Human, mouse, rat, dog, rabbit, pig, cat, chicken, sea urchin, chlamydomonas. Does not react with bovine and yeast.
Applications
WB, IF, IP, IHC
Pricing
$280/0.1 mg | $985/5x0.1 mg
Western Blot (WB)
(Western blot analysis of hsc70 using various lysates.Lanes 1 - 2: Merged signal (red and green). Green - AF5187 observed at 70kDa. Red - loading control, MBS9610328, observed at 55 kDa. Blots were developed with Goat Anti-Rabbit IgG(H+L)FITC–conjugated (MBS9612520) and Goat Anti-Mouse IgG(H+L) AlexaFluor 594–conjugated (MBS9612517) secondary antibodies)
beta-Tubulin, Monoclonal Antibody (Cat# AAA9610328)
Full Name
beta-Tubulin Mouse Monoclonal Antibody
Gene Names
TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
Applications
WB, IHC, IF, ICC
Purity
Peptide affinity purification
Pricing
$190/0.05 mL | $245/0.1 mL | $310/0.2 mL | $855/1 mL | $3,800/5x1 mL
Western Blot (WB)
(JMJD3 Antibody)
JMJD3, Antibody (Cat# AAA150629)
Full Name
JMJD3 Antibody
Gene Names
KDM6B; JMJD3
Reactivity
Human, Mouse, Rat
Applications
EIA, ICC, IF, WB
Purity
JMJD3 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded R. brain section using CB2 Antibody(C-term). MBS9203911 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
CB2, Polyclonal Antibody (Cat# AAA9203911)
Full Name
CB2 Antibody (C-term)
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Human
Applications
EIA, IHC, FC/FACS, WB
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
MBP Tag, Antibody (Cat# AAA355033)
Full Name
MBP tag antibody
Reactivity
All species
Applications
WB
Pricing
$280/0.1 mg | $985/5x0.1 mg
ATF6, Polyclonal Antibody (Cat# AAA150934)
Full Name
ATF6 Antibody
Gene Names
ATF6; ATF6A
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
ATF6 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation in Cell Lines)
DR4, Polyclonal Antibody (Cat# AAA151351)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
EIA, WB
Purity
DR4 Antibody is Antibody is DEAE purified.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
S1 Tag, Antibody (Cat# AAA355017)
Full Name
S1 tag antibody
Reactivity
All species
Applications
WB
Pricing
$325/0.1 mg | $1,185/5x0.1 mg
DYKDDDDK Tag, Antibody (Cat# AAA355038)
Full Name
DYKDDDDK tag antibody
Reactivity
All species
Applications
WB, IF, IP
Pricing
$280/0.1 mg | $985/5x0.1 mg
beta-Tubulin mAb, Antibody (Cat# AAA355045)
Full Name
beta-Tubulin antibody
Reactivity
Human, mouse, rat, monkey, Chinese hamster, dog
Applications
WB, IF, EIA
Pricing
$280/0.1 mg | $985/5x0.1 mg
beta-Actin mAb, Antibody (Cat# AAA355034)
Full Name
beta-Actin antibody
Reactivity
Human, mouse, rat, bovine, sheep, pig, rabbit, cat, dog, guinea pig, chicken, carp, leech
Applications
WB, IF, IP, EIA, IHC
Pricing
$280/0.1 mg | $985/5x0.1 mg
Testing Data
(Western blot analysis of COS-7 simian fibroblast-like whole cell lysate probed with Mouse anti p53 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence)
p53, Monoclonal Antibody (Cat# AAA212262)
Full Name
MOUSE ANTI p53 (aa20-25)
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Bovine, Cat, Green Monkey, Horse
Applications
EIA, IP, WB
Purity
Purified IgG prepared by affinity chromatography on Protein A
Pricing
$345/0.1 mg | $1,405/5x0.1 mg
Actin mAb, Antibody (Cat# AAA355012)
Full Name
Actin antibody
Reactivity
Human, mouse, rat, monkey, Chinese hamster, dog, spodoptera frugiperda
Applications
WB
Pricing
$280/0.1 mg | $985/5x0.1 mg
His Tag, Antibody (Cat# AAA355015)
Full Name
His tag antibody
Reactivity
All species
Applications
WB, IF, IP, EIA
Pricing
$280/0.1 mg | $985/5x0.1 mg
V5 Tag, Antibody (Cat# AAA355028)
Full Name
V5 tag antibody
Reactivity
All species
Applications
WB, IF, IP
Pricing
$280/0.1 mg | $985/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
TIM-1, Polyclonal Antibody (Cat# AAA150916)
Full Name
TIM-1 Antibody
Gene Names
HAVCR1; TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
TIM-1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
beta-Tubulin mAb, Antibody (Cat# AAA355046)
Full Name
beta-Tubulin antibody
Reactivity
Human, mouse, rat
Applications
WB, IF, IHC
Pricing
$280/0.1 mg | $985/5x0.1 mg
GST Tag, Antibody (Cat# AAA355039)
Full Name
GST tag antibody
Reactivity
All species
Applications
WB, EIA
Pricing
$280/0.1 mg | $985/5x0.1 mg
HA Tag, Antibody (Cat# AAA355030)
Full Name
HA tag antibody
Reactivity
All species
Applications
WB, IF, IP, EIA
Pricing
$280/0.1 mg | $985/5x0.1 mg
Mcherry Tag, Antibody (Cat# AAA355025)
Full Name
mCherry tag antibody
Reactivity
All species
Applications
WB
Pricing
$325/0.1 mg | $1,185/5x0.1 mg
GFP Tag, Antibody (Cat# AAA355040)
Full Name
GFP tag antibody
Reactivity
All species
Applications
WB, IF, IP, EIA
Pricing
$280/0.1 mg | $985/5x0.1 mg
CBP Tag, Antibody (Cat# AAA355043)
Full Name
CBP Tag antibody
Reactivity
All species
Applications
WB, EIA
Pricing
$280/0.1 mg | $985/5x0.1 mg
GAPDH mAb, Antibody (Cat# AAA355026)
Full Name
GAPDH antibody
Gene Names
Gapdh; Gapd
Reactivity
Human, mouse, rat, dog, rabbit, pig, chicken. Does not react with non-vertebrate and prokaryote species.
Applications
WB, IF, IP, IHC
Pricing
$280/0.1 mg | $985/5x0.1 mg
GAPDH mAb, Antibody (Cat# AAA355044)
Full Name
GAPDH antibody
Gene Names
Gapdh; Gapd
Reactivity
Human, monkey
Applications
WB, IF, EIA
Pricing
$280/0.1 mg | $985/5x0.1 mg
c-Myc Tag, Antibody (Cat# AAA355018)
Full Name
c-Myc tag antibody
Reactivity
All species
Applications
WB, IF, IP
Pricing
$280/0.1 mg | $985/5x0.1 mg
Western Blot (WB)
(Western blot detection of MEK-2 in THP-1, Jurkat, Raji, 3T3 and Hela cell lysates using 310001 (MEK-2 rabbit pAb, 1:1000 diluted), then incubated with 201069 (Rabbit IgG mAb, 1:50000 diluted).Anti-mouse IgG, HRP-linked Antibody was used for detecting.Predicted band size:44KDa.Observed band size:44KDa.)
IgG, Monoclonal Antibody (Cat# AAA475172)
Full Name
Anti-Rabbit IgG Mouse mAb
Reactivity
Rat
Applications
WB, EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA214958)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210664)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Western Blot (WB)
(Western blot analysis of RAN Antibody (N-term) in HL-60 cell line lysates (35ug/lane).RAN (arrow) was detected using the purified Pab.)
RAN, Polyclonal Antibody (Cat# AAA9210295)
Full Name
RAN Antibody (N-term)
Gene Names
RAN; TC4; Gsp1; ARA24
Reactivity
Human, mouse (Predicted Reactivity: Yeast, Bovine, C Elegans, Chicken, Zebrafish, Monkey, Rat, Xenopus)
Applications
WB, EIA, IHC-p, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA214268)
Full Name
RAT ANTI MOUSE CD8:Low Endotoxin
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS, IF
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
