Typical Testing Data/Standard Curve (for reference only)
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Porcine Alpha-1-acid glycoprotein 1 (ORM1) ELISA Kit | ORM1 elisa kit
Porcine Alpha-1-acid glycoprotein 1 (ORM1) ELISA Kit
Gene Names
ORM1; ORM; AGP1; AGP-A; HEL-S-153w
Reactivity
Porcine
Synonyms
Alpha-1-acid glycoprotein 1 (ORM1); Porcine Alpha-1-acid glycoprotein 1 (ORM1) ELISA Kit; ORM1 elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of ORM1. No significant cross-reactivity or interference between ORM1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ORM1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 mg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for ORM1 elisa kit
Intended Uses: This ORM1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ORM1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: ORM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ORM1 antibody and an ORM1-HRP conjugate. The assay sample and buffer are incubated together with ORM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ORM1 concentration since ORM1 from samples and ORM1-HRP conjugate compete for the anti-ORM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ORM1 from the sample, fewer sites are left to bind ORM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ORM1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ORM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ORM1 antibody and an ORM1-HRP conjugate. The assay sample and buffer are incubated together with ORM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ORM1 concentration since ORM1 from samples and ORM1-HRP conjugate compete for the anti-ORM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ORM1 from the sample, fewer sites are left to bind ORM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ORM1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ORM1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,512 Da
NCBI Official Full Name
alpha-1-acid glycoprotein 1
NCBI Official Synonym Full Names
orosomucoid 1
NCBI Official Symbol
ORM1
NCBI Official Synonym Symbols
ORM; AGP1; AGP-A; HEL-S-153w
NCBI Protein Information
alpha-1-acid glycoprotein 1; AGP 1; OMD 1; orosomucoid-1; epididymis secretory sperm binding protein Li 153w
UniProt Protein Name
Alpha-1-acid glycoprotein 1
Protein Family
UniProt Gene Name
ORM1
UniProt Synonym Gene Names
AGP1; AGP 1; OMD 1
UniProt Entry Name
A1AG1_HUMAN
NCBI Description
This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. [provided by RefSeq, Jul 2008]
Uniprot Description
ORM1: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9q32
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding
Biological Process: regulation of immune system process; transport; acute-phase response; inflammatory response
Research Articles on ORM1
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Product Notes
The Porcine ORM1 orm1 (Catalog #AAA7247283) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7247283 ELISA Kit recognizes Porcine ORM1. It is sometimes possible for the material contained within the vial of "Alpha-1-acid glycoprotein 1 (ORM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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