Mouse anti-All species HA Tag Antibody | anti-HA antibody
HA tag antibody
Reactivity
All species
Applications
Western Blot, Immunofluorescence, Immunoprecipitation, ELISA
Synonyms
HA Tag; HA tag antibody; Monoclonal Anti-HA Tag; anti-HA antibody
Host
Mouse
Reactivity
All species
Isotype
IgG1
Specificity
Detect over-expressed proteins containing the HA epitope tag.
Form/Format
Liquid (1mg/ml)
Applicable Applications for anti-HA antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
WB (1:5000), IP (1:100), IF (1:1000), ELISA (1:2000)
Immunogen
A synthetic peptide YPYDVPDYA conjugated to KLH.
Preparation and Storage
Store at -20 degree C for 1 year. Or aliquot and store at -80 degree C for long term. Avoid repeated freeze and thaw cycles.
Related Product Information for anti-HA antibody
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. The vectors have been engineered so that the GST carrier can be cleaved from fusion proteins by digestion with site-specific proteases such as thrombin or blood coagulation factor Xa, following which, the carrier and any uncleaved fusion protein can be removed by absorption on glutathione-agarose. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Product Categories/Family for anti-HA antibody
References
1. Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. 1988, 67(1):31-40. PMID:3047011
NCBI and Uniprot Product Information
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Product Notes
The HA (Catalog #AAA355030) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HA tag antibody reacts with All species and may cross-react with other species as described in the data sheet. AAA Biotech's HA Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA). WB (1:5000), IP (1:100), IF (1:1000), ELISA (1:2000). Researchers should empirically determine the suitability of the HA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA Tag, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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