Mouse beta-Actin mAb Antibody
beta-Actin antibody
Reactivity
Human, mouse, rat, bovine, sheep, pig, rabbit, cat, dog, guinea pig, chicken, carp, leech
Applications
Western Blot, Immunofluorescence, Immunoprecipitation, ELISA, Immunohistochemistry
Synonyms
beta-Actin mAb; beta-Actin antibody; Monoclonal Anti- beta-Actin ; Monoclonal Anti- b-Actin; anti-beta-Actin mAb antibody
Host
Mouse
Reactivity
Human, mouse, rat, bovine, sheep, pig, rabbit, cat, dog, guinea pig, chicken, carp, leech
Isotype
IgG1
Specificity
Detect the epitope located on N-terminal of beta-Actin.
Form/Format
Liquid
Applicable Applications for anti-beta-Actin mAb antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Western Blot (1:100-1000)
Immunoprecipitation, IP (1-2 ug per 100-500 ug of total protein. 1ml of cell lysate)
Immunoprecipitation, IP (1-2 ug per 100-500 ug of total protein. 1ml of cell lysate)
Immunogen
Gizzard Actin of chicken origin.
Preparation and Storage
Store at -20 degree C for 1 year. Avoid repeated freeze and thaw cycles.
Related Product Information for anti-beta-Actin mAb antibody
Beta-actin (gene name ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Karakozova et al. (2006) found that approximately 40% of intracellular beta-actin is arginylated in vivo. In both wildtype and Ate1-null cells beta-actin was stable, suggesting that arginylation does not induce beta-actin degradation. And arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.1 Nitric oxide (NO) is a paracrine mediator of vascular and platelet function that is produced in the vasculature by NO synthase-3. And beta-actin plays a critical role in regulating NO formation and signaling in platelets.2 Beta actin is also usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western Blotting.
Product Categories/Family for anti-beta-Actin mAb antibody
References
1. Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., Kashina, A. Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313: 192-196, 2006. 2. Ji, Y., Ferracci, G., Warley, A., Ward, M., Leung, K.-Y., Samsuddin, S., Leveque, C., Queen, L., Reebye, V., Pal, P., Gkaliagkousi, E., Seager, M., Ferro, A. Beta-actin regulates platelet nitric oxide synthase 3 activity through interaction with heat shock protein 90. Proc. Nat. Acad. Sci. 104: 8839-8844, 2007
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Product Notes
The beta-Actin mAb (Catalog #AAA355034) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta-Actin antibody reacts with Human, mouse, rat, bovine, sheep, pig, rabbit, cat, dog, guinea pig, chicken, carp, leech and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Actin mAb can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA), Immunohistochemistry (IHC). Western Blot (1:100-1000) Immunoprecipitation, IP (1-2 ug per 100-500 ug of total protein. 1ml of cell lysate). Researchers should empirically determine the suitability of the beta-Actin mAb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin mAb, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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