Mouse GAPDH mAb Antibody | anti-GAPDH antibody

GAPDH antibody

Cat. Num. AAA355044

• Gene Names: Gapdh; Gapd
• Reactivity: Human, monkey
• Applications: Western Blot, Immunofluorescence, ELISA
• Synonyms: GAPDH mAb; GAPDH antibody; Monoclonal Anti- GAPDH; anti-GAPDH antibody

• Host: Mouse
• Reactivity: Human, monkey
• Isotype: IgG1
• Specificity: Detect the endogenous levels of total GAPDH protein.
• Form/Format: Liquid

• Applicable Applications for anti-GAPDH antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: Western Blot (WB): (1:5000); Immunofluorescence (IF): (1:500); ELISA (EIA): (1:1000-2000); Other applications have not been tested.; The optimal dilutions should be determined by end user.
• Immunogen: A synthetic peptide (conjugated to KLH) corresponding to the C-terminal of human GAPDH.
• Preparation and Storage: Store at -20 degree C for 1 year. Avoid repeated freeze and thaw cycles.

Related Product Information for anti-GAPDH antibody

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.

Product Categories/Family for anti-GAPDH antibody

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References

1. Meyer-Siegler, K., Mauro, D. J., Seal, G., Wurzer, J., deRiel, J. K., Sirover, M. A. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proc. Nat. Acad. Sci. 88: 8460-8464, 1991. 2. Zheng L, Roeder RG, Luo Y (2003). "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component". Cell 114 (2): 255-66. 3. A. Tarze, A. Deniaud, M. Le Bras, E. Maillier, D. Molle, N. Larochette, N. Zamzami, G. Jan, G. Kroemer, and C. Brenner (2007). "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization". Oncogene 26 (18): 2606-2620.

NCBI and Uniprot Product Information

• NCBI GI #: 122065190
• NCBI GeneID: 24383
• UniProt Accession #: P04797; P09328; Q5M916; Q9QWU4
• Molecular Weight: 35,828 Da
• NCBI Official Full Name: Glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: Gapdh
• NCBI Official Synonym Symbols: Gapd
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase; BARS-38; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: Gapdh
• UniProt Synonym Gene Names: Gapd; GAPDH
• UniProt Entry Name: G3P_RAT

NCBI Description

catalyzes the interconversion of D-glyceraldehyde 3-phosphate, phosphate, and NAD(+) to 3-phospho-D-glyceroyl phosphate and NADH; may contribute to apoptosis [RGD, Feb 2006]

Uniprot Description

Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation

By similarity. Ref.3 Ref.12 Ref.13 Ref.15

Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure: Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct

By similarity. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules. Component of the GAIT complex

By similarity. Ref.12 Ref.13 Ref.14 Ref.15

Subcellular location: Cytoplasm › cytosol. Cytoplasm › cytoskeleton. Nucleus. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Colocalizes with CHP1 to small punctate structures along the microtubules tracks. Ref.12 Ref.13 Ref.14 Ref.15

Tissue specificity: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Ref.8

Post-translational modification: S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus. The effect of S-nitrosylation on enzymatic activity is unclear: according to some authors, it inhibits enzymatic activity and increases endogenous ADP-ribosylation, inhibiting the enzyme in a non-reversible manner (Ref.11). According to others, it does not affect glycolysis (Ref.13). ADP-ribosylation is likely to be a pathophysiological event associated with inhibition of gluconeogenesis.ISGylated

By similarity.Sulfhydration at Cys-150 increases catalytic activity

By similarity.

Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.