Mouse anti-All species His Tag Antibody
His tag antibody
Reactivity
All species
Applications
Western Blot, Immunofluorescence, Immunoprecipitation, ELISA
Synonyms
His Tag; His tag antibody; Monoclonal Anti-His Tag; anti-His Tag antibody
Host
Mouse
Reactivity
All species
Isotype
IgG1
Specificity
Detect over-expressed or recombinant proteins containing the 6 × His epitope tag.
Form/Format
Liquid (1mg/ml)
Applicable Applications for anti-His Tag antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
WB (1:5000), IP (1:100), IF (1:2000), ELISA (1:2000)
Immunogen
A synthetic peptide 6 × His conjugated to KLH.
Preparation and Storage
Store at -20 degree C for 1 year. Or aliquot and store at -80 degree C for long term. Avoid repeated freeze and thaw cycles
Related Product Information for anti-His Tag antibody
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Affinity purification using a polyhistidine-tag usually results in relatively pure protein when the recombinant protein is expressed in prokaryotic organisms. Depending on downstream applications including the purification of protein complexes to study protein interactions, purification from higher organisms such as yeasts or other eukaryotes may require a tandem affinity purification using two tags to yield higher purity. The most common polyhistidine tags are formed of six histidine (6xHis tag) residues - preceded by Methionine - which are added at the C-terminal or N-terminal of the protein of interest.
Product Categories/Family for anti-His Tag antibody
References
1. Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285-6. 2. Gavin, AC; Bösche, M; Krause, R; Grandi, P; Marzioch, M; Bauer, A; Schultz, J; Rick, JM et al. (2002). "Functional organization of the yeast proteome by systematic analysis of protein complexes". Nature 415 (6868): 141-7.
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Product Notes
The His Tag (Catalog #AAA355015) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The His tag antibody reacts with All species and may cross-react with other species as described in the data sheet. AAA Biotech's His Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA). WB (1:5000), IP (1:100), IF (1:2000), ELISA (1:2000). Researchers should empirically determine the suitability of the His Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His Tag, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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