Rabbit Cyclin A1 Polyclonal Antibody | anti-A1 antibody

Cyclin A1 (R436) Polyclonal Antibody

Cat. Num. AAA3000444

• Gene Names: CCNA1; CT146
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cyclin A1; Polyclonal Antibody; Cyclin A1 (R436) Polyclonal Antibody; Cyclin-A1; CCNA1; CyclinA1; anti-A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cyclin A1 (R436) polyclonal antibody detects endogenous levels of Cyclin A1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 464

• Applicable Applications for anti-A1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human Cyclin A1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Cyclin A1 (R436) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-A1 antibody

Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.

NCBI and Uniprot Product Information

• NCBI GI #: 161377468
• NCBI GeneID: 8900
• NCBI Accession #: NP_001104515.1
• NCBI GenBank Nucleotide #: NP_001104515.1
• UniProt Accession #: P78396
• Molecular Weight: ~ 52kDa
• NCBI Official Full Name: cyclin-A1 isoform b
• NCBI Official Synonym Full Names: cyclin A1
• NCBI Official Symbol: CCNA1
• NCBI Official Synonym Symbols: CT146
• NCBI Protein Information: cyclin-A1
• UniProt Protein Name: Cyclin-A1
• Protein Family: Protein
• UniProt Gene Name: CCNA1
• UniProt Entry Name: CCNA1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNA1: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Belongs to the cyclin family. Cyclin AB subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Cancer Testis Antigen (CTA); Cell cycle regulation

Chromosomal Location of Human Ortholog: 13q12.3-q13

Cellular Component: nucleoplasm; microtubule cytoskeleton; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; mitosis; male meiosis I; cell division; regulation of G2/M transition of mitotic cell cycle; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle