ALCAM / CD166 recombinant protein

Recombinant Rat ALCAM / CD166 Protein (His tag)

Cat. Num. AAA2547414

• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: ALCAM / CD166; Recombinant Rat ALCAM / CD166 Protein (His tag); ALCAM; ALCAM / CD166 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 583

• Application Notes: The recombinant rat ALCAM comprises 511 amino acids and predicts a molecular mass of 57.5 kDa. The apparent molecular mass of the recombinant protein is approximately 68-91 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Trp 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ALCAM / CD166 recombinant protein

Background: Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

Description: A DNA sequence encoding the rat ALCAM (O35112) (Met1-Lys527) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 13929058
• NCBI GeneID: 79559
• NCBI Accession #: NP_113941.1
• NCBI GenBank Nucleotide #: NM_031753.1
• UniProt Accession #: O35112; O55172
• Molecular Weight: 65,022 Da
• NCBI Official Full Name: CD166 antigen
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: Alcam
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• UniProt Gene Name: Alcam

Uniprot Description

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (). Mediates outgrowth and pathfinding for retinal ganglion cell axons ().