Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) RTU ELISA Kit | ALCAM rtu elisa kit

Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

Cat. Num. AAA457527

• Reactivity: Rat
• Synonyms: Activated Leukocyte Cell Adhesion Molecule (ALCAM); Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit; CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule; ALCAM rtu elisa kit

• Reactivity: Rat
• Sequence Length: 583

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALCAM rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALCAM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ALCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ALCAM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13929058
• NCBI GeneID: 79559
• NCBI Accession #: NP_113941.1
• NCBI GenBank Nucleotide #: NM_031753.1
• UniProt Accession #: O35112; O55172
• Molecular Weight: 65,022 Da
• NCBI Official Full Name: CD166 antigen
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: Alcam
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: Alcam

Uniprot Description

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (). Mediates outgrowth and pathfinding for retinal ganglion cell axons ().