Rat CD166 ELISA Kit | ALCAM elisa kit

Rat CD166 ELISA Kit

Cat. Num. AAA8248468

• Reactivity: Rat
• Synonyms: CD166; Rat CD166 ELISA Kit; MEMD; CD166 antigen; Activated leukocyte cell adhesion molecule; ALCAM elisa kit

• Reactivity: Rat
• Specificity: The Rat CD166 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CD166 proteins within the range of 78 pg/ml - 5000 pg/ml.
• Sequence Length: 583

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALCAM elisa kit

Principle of the Assay: The Rat CD166 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CD166 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Rat CD166 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD166 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CD166 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD166 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The human gene was mapped to chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. This gene is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

NCBI and Uniprot Product Information

• NCBI GI #: 13929058
• NCBI GeneID: 79559
• NCBI Accession #: NP_113941.1
• NCBI GenBank Nucleotide #: NM_031753.1
• UniProt Accession #: O35112; O55172
• Molecular Weight: 65,022 Da
• NCBI Official Full Name: CD166 antigen
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: Alcam
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: Alcam
• UniProt Entry Name: CD166_RAT

Uniprot Description

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (). Mediates outgrowth and pathfinding for retinal ganglion cell axons ().