Rabbit RIPK3 Polyclonal Antibody | anti-RIPK3 antibody

RIPK3 Polyclonal Antibody

Cat. Num. AAA3011012

• Gene Names: RIPK3; RIP3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RIPK3; Polyclonal Antibody; RIPK3 Polyclonal Antibody; Receptor-interacting serine/threonine-protein kinase 3; RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3; RIP3; anti-RIPK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RIP3 polyclonal antibody detects endogenous levels of Receptor-interacting serine/threonine-protein kinase 3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 518

• Applicable Applications for anti-RIPK3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human RIP3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of RIPK3 antibody.)

Related Product Information for anti-RIPK3 antibody

The death domain is a cytoplasmic domain of approximately 80 amino acids that is necessary for the transduction of apoptotic signals and is present in the apoptosis-mediating receptors TNF-R1 and FAS. Other death domain-containing, but otherwise structurally unrelated proteins have been identified on the basis of their ability to associate with the cytoplasmic domains of TNF-R1 or FAS. One of these proteins, the receptor-interacting protein 3 (RIP3), contains an N-terminal kinase domain and shares extensive homology with RIP and RIP2. However, RIP3 contains a unique C-terminal death domain, which promotes apoptosis. RIP3 can be expressed as two splice variants, RIP3beta and RIP3gamma, which contain a truncated N-terminal kinase domain and a distinct and shorter C-terminus. Subsequently, expression of these splice variants downregulates RIP3-mediated apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 205371831
• NCBI GeneID: 11035
• UniProt Accession #: Q9Y572
• Molecular Weight: ~ 45-70kDa
• NCBI Official Full Name: Receptor-interacting serine/threonine-protein kinase 3
• NCBI Official Synonym Full Names: receptor interacting serine/threonine kinase 3
• NCBI Official Symbol: RIPK3
• NCBI Official Synonym Symbols: RIP3
• NCBI Protein Information: receptor-interacting serine/threonine-protein kinase 3
• UniProt Protein Name: Receptor-interacting serine/threonine-protein kinase 3
• Protein Family: Receptor-interacting serine/threonine-protein kinase
• UniProt Gene Name: RIPK3
• UniProt Synonym Gene Names: RIP3; RIP-3
• UniProt Entry Name: RIPK3_HUMAN

NCBI Description

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RIPK family

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrion; plasma membrane; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; identical protein binding; protein binding; transcription coactivator activity; protein complex binding; ATP binding; protein kinase activity

Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; regulation of activated T cell proliferation; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; MyD88-independent toll-like receptor signaling pathway; T cell differentiation in the thymus; protein modification process; toll-like receptor 3 signaling pathway; signal transduction; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; toll-like receptor signaling pathway; positive regulation of interferon type I production; activation of protein kinase activity; innate immune response; T cell homeostasis; positive regulation of ligase activity; toll-like receptor 4 signaling pathway; protein homooligomerization; positive regulation of oxidoreductase activity