Testing Data
(SDS PAGE.)
AHA1 recombinant protein
Recombinant Yeast AHA1
Reactivity
Yeast
Applications
Western Blot
Purity
>90%
Synonyms
Aha1; Recombinant Yeast AHA1; SHSA1; HspC322; p38; AHA1 recombinant protein
Host
Yeast
Reactivity
Yeast
Purity/Purification
>90%
Form/Format
Affinity Purified
Concentration
1.8mg/mL (varies by lot)
Sequence Length
350
Applicable Applications for AHA1 recombinant protein
Western Blot (WB) control, functional/activity studies
Certificate of Analysis
This product has been certified >90% pure using SDS - PAGE analysis.
Conjugate Tag
His tag
Expression System
E Coli
Storage Buffer
25mM Tris buffer pH7.2, 150mM NaCl, 0.1mM DTT, 50% glycerol
Preparation and Storage
-20 degree C
Testing Data
(SDS PAGE.)
Testing Data
(SDS PAGE.)
Testing Data #2
(ATPase assay using yeast Hsp82 activated by. Reaction conditions are 25mM Hepes pH7.2, 2.2mM NaCl, 5mM MgCl2, 2mM ATP, 1mM PEP, 1mM DTT, PK/LDH, 300uM NADH)
Testing Data #2
(ATPase assay using yeast Hsp82 activated by. Reaction conditions are 25mM Hepes pH7.2, 2.2mM NaCl, 5mM MgCl2, 2mM ATP, 1mM PEP, 1mM DTT, PK/LDH, 300uM NADH)
Related Product Information for AHA1 recombinant protein
Scientific Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
Product Categories/Family for AHA1 recombinant protein
References
1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~40.5kDa
NCBI Official Full Name
Aha1p
NCBI Official Symbol
AHA1
NCBI Protein Information
Aha1p
UniProt Protein Name
Hsp90 co-chaperone AHA1
Protein Family
UniProt Gene Name
AHA1
UniProt Synonym Gene Names
YDR214W; YD8142.16; YD8142B.06
UniProt Entry Name
AHA1_YEAST
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Product Notes
The AHA1 aha1 (Catalog #AAA806931) is a Recombinant Protein produced from Yeast and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Yeast AHA1 reacts with Yeast and may cross-react with other species as described in the data sheet. AAA Biotech's Aha1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) control, functional/activity studies. Researchers should empirically determine the suitability of the AHA1 aha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aha1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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