Western Blot (WB)
(Western Blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1,000 dilution.)
Rabbit anti-Human HIF1AN Polyclonal Antibody | anti-HIF1AN antibody
HIF1AN Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
HIF1AN; Polyclonal Antibody; HIF1AN Polyclonal Antibody; FIH-1; FIH1; DKFZp762F1811; Factor inhibiting HIF-1; Factor inhibiting HIF1; FIH 1; FLJ20615; FLJ22027; HIF1N; Hypoxia inducible factor 1 alpha inhibitor; Hypoxia inducible factor 1 alpha subunit inhibitor; Hypoxia inducible factor asparagine hydroxylase; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; Peptide aspartate beta dioxygenase; anti-HIF1AN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
HIF1AN polyclonal antibody detects endogenous levels of HIF1AN protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-HIF1AN antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein within human HIF1AN aa 1-200.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1,000 dilution.)
Western Blot (WB)
(Western Blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1,000 dilution.)
Immunocytochemistry (ICC)
(ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-HIF1AN antibody
Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like beta-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
40kDa
Protein Family
Similar Products
Product Notes
The HIF1AN (Catalog #AAA3012816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIF1AN Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIF1AN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the HIF1AN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF1AN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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