Rabbit anti-Mouse, Rat MCP-1 Polyclonal Antibody | anti-MCP-1 antibody

MCP-1 Antibody

Cat. Num. AAA840399

• Gene Names: Ccl2; MCP-1; Scya2; Sigje
• Reactivity: Mouse, Rat
• Applications: Western Blot, Neutralization Assay
• Purity: Affinity purified
• Synonyms: MCP-1; Polyclonal Antibody; MCP-1 Antibody; MCP1; MCP 1; monocyte chemoattracctant protein-1; monocyte chemoattracctant protein 1; monocyte chemoattracctant protein; anti-MCP-1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) immunoaffinity purified rabbit anti-rat MCP-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 148

• Applicable Applications for anti-MCP-1 antibody: Western Blot (WB), Neutralization
• Application Notes: Western blot analysis (0.5-2 ug/ml). From researcher's feedback, it can also be used for neutralization studies. However, the optimal conditions should be determined individually.
• Immunogen: E. coli-expressed rat MCP-1 (a.a. 1-73)
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-MCP-1 antibody

Background: Rat MCP-1/JE (monocyte chemoattractant protein-1) is a 148-amino acid CC chemokine with a N-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con-A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3'-end. The 3'-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and which explains for the higher molecular weight (25 kDa). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophiles. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.

References

Role of atrial endothelial cells in the development of atrial fibrosis and fibrillation in response to pressure overload . Osamu Kume, Yasushi Teshima, Ichitaro Abe, Yuki Ikebe, Takahiro Oniki, Hidekazu Kondo, Shotaro Saito, Akira Fukui, Kunio Yufu, Masahiro Miura, Tatsuo Shimada, Naohiko Takahashi (2016)

NCBI and Uniprot Product Information

• NCBI GI #: 13928714
• NCBI GeneID: 24770
• NCBI Accession #: NP_113718.1
• NCBI GenBank Nucleotide #: NM_031530.1
• UniProt Accession #: P14844; Q549R5
• Molecular Weight: 16,460 Da
• NCBI Official Full Name: C-C motif chemokine 2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 2
• NCBI Official Symbol: Ccl2
• NCBI Official Synonym Symbols: MCP-1; Scya2; Sigje
• NCBI Protein Information: C-C motif chemokine 2
• UniProt Protein Name: C-C motif chemokine 2
• Protein Family: Major capsid protein
• UniProt Gene Name: Ccl2
• UniProt Synonym Gene Names: Je; Mcp1; Scya2; MCP-1
• UniProt Entry Name: CCL2_RAT

NCBI Description

a monocyte chemoattractant protein [RGD, Feb 2006]

Uniprot Description

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine; Secreted

Cellular Component: extracellular space; cell soma; rough endoplasmic reticulum; perinuclear region of cytoplasm; endocytic vesicle; dendrite; cytoplasm; extracellular region; perikaryon; synapse; nerve terminal

Molecular Function: heparin binding; G-protein-coupled receptor binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: maternal process involved in pregnancy; positive regulation of cell adhesion; positive regulation of leukocyte migration; response to glucocorticoid stimulus; response to lipopolysaccharide; response to antibiotic; monocyte chemotaxis; regulation of cell shape; transforming growth factor beta receptor signaling pathway; response to vitamin B3; negative regulation of neuron apoptosis; response to drug; neutrophil chemotaxis; organ regeneration; positive regulation of tumor necrosis factor production; response to amino acid stimulus; response to ethanol; response to bacterium; cellular response to insulin stimulus; response to heat; response to mechanical stimulus; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; chemokinesis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; lipopolysaccharide-mediated signaling pathway; positive regulation of cell-cell adhesion; protein kinase B signaling cascade; response to wounding; response to gamma radiation; inflammatory response; lymphocyte chemotaxis; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; glial cell migration; macrophage chemotaxis; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; negative regulation of angiogenesis; positive regulation of leukocyte mediated cytotoxicity; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration