Rat IL-1beta CLIA Kit | IL-1beta clia kit

IL-1beta (Rat) LumiAb ELISA Kit

Cat. Num. AAA9510861

• Gene Names: Il1b; IL-1F2
• Reactivity: Rat
• Synonyms: IL-1beta; IL-1beta (Rat) LumiAb ELISA Kit; Rat IL-1beta; Interleukin-1 beta; IL-1 beta; IL-1beta clia kit

• Reactivity: Rat
• Specificity: The LumiAb Rat IFN-gamma ELISA is capable of recognizing both recombinant and naturally produced Rat IFN-gamma proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 5% cross reactivity.
• Sequence Length: 268

• Assay Type: Quantitative Sandwich
• Sensitivity: 5 pg/ml.
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-1beta clia kit

Principle of the Assay: The LumiAb Rat IFN-gamma Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Rat IFN-gamma concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Rat IFN-gamma while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Rat IFN-gamma or Interferon-gamma is a 156 amino acid cytokine encoded by the Ifng gene located at locus 7q22 on chromosome 7. After initial synthesis of the peptide, the 22 residue signal sequence is cleaved, allowing the 134 residue IFN-gamma protein to fold properly. Produced by lymphocytes activated by specific antigens or mitogens, IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. The homodimeric cytokine of the type gamma interferon family is a potent activator of macrophages, has anti-proliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. IFN-gamma is primarily released from activated T-cells.

Product Categories/Family for IL-1beta clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 2497335
• NCBI GeneID: 24494
• UniProt Accession #: Q63264
• NCBI Official Full Name: Interleukin-1 beta
• NCBI Official Synonym Full Names: interleukin 1 beta
• NCBI Official Symbol: Il1b
• NCBI Official Synonym Symbols: IL-1F2
• NCBI Protein Information: interleukin-1 beta
• UniProt Protein Name: Interleukin-1 beta
• UniProt Gene Name: Il1b
• UniProt Synonym Gene Names: IL-1 beta
• UniProt Entry Name: IL1B_RAT

NCBI Description

an inflammatory cytokine [RGD, Feb 2006]

Uniprot Description

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family.

Protein type: Cytokine

Cellular Component: extracellular space; extracellular region; vesicle; secretory granule

Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of JNK activity; negative regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; negative regulation of glutamate secretion; glycoprotein metabolic process; positive regulation of apoptosis; activation of MAPK activity; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; germ cell programmed cell death; response to glucocorticoid stimulus; negative regulation of insulin receptor signaling pathway; positive regulation of NF-kappaB import into nucleus; positive regulation of glial cell differentiation; positive regulation of lipid catabolic process; response to lipopolysaccharide; fever; response to organic cyclic substance; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; elevation of cytosolic calcium ion concentration; response to vitamin D; pentacyclic triterpenoid metabolic process; positive regulation of phagocytosis; positive regulation of T cell proliferation; positive regulation of astrocyte differentiation; response to drug; neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; interleukin-1 beta production; positive regulation of interleukin-6 production; social behavior; response to organic nitrogen; purine base metabolic process; negative regulation of neuron differentiation; positive regulation of angiogenesis; response to ethanol; response to heat; positive regulation of cell division; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of lipid metabolic process; leukocyte migration; response to peptide hormone stimulus; estrogen metabolic process; sequestering of triacylglycerol; wound healing; positive regulation of interleukin-6 biosynthetic process; response to morphine; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; response to L-ascorbic acid; chronic inflammatory response to antigenic stimulus; response to estradiol stimulus; positive regulation of stress-activated MAPK cascade; negative regulation of neurogenesis; positive regulation of interleukin-8 production; negative regulation of cell proliferation; learning and/or memory; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; response to gamma radiation; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; response to nutrient; aging; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of immature T cell proliferation in the thymus; memory; response to ATP; ovulation; polyketide metabolic process; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; response to ozone; positive regulation of prostaglandin secretion; response to hypoxia; positive regulation of fever; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion