Western Blot (WB)
(Anti-ZNF823 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:68 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human ZNF823 Polyclonal Antibody | anti-ZNF823 antibody
Anti-ZNF823 Antibody, Rabbit Polyclonal
Gene Names
ZNF823; HSZFP36
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
ZNF823; Polyclonal Antibody; Anti-ZNF823 Antibody; Rabbit Polyclonal; ZNF823 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-HSZFP36 Antibody; anti-ZNF823 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ZNF823
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ZNF823 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IP: 1-2uL/mg of lysate
IP: 1-2uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human ZNF823
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human ZNF823, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ZNF823 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:68 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-ZNF823 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:68 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(ZNF823 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-ZNF823 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ZNF823 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 67 kDaObserved band size: 75 kDa)
Immunoprecipitation (IP)
(ZNF823 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-ZNF823 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ZNF823 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 67 kDaObserved band size: 75 kDa)
Related Product Information for anti-ZNF823 antibody
ZNF823 is also known as zinc finger protein 823, HSZFP36, and ZFP 36 for a zinc finger protein. It is widely accepted that ZNF823 is involved in transcriptional regulation. ZNF823 can binding various molecules such as metal ion and sequence-specific DNA. It also regulates the activity of RNA polymerase II transcription factor. Moreover, ZNF823 was identified as one of seven master regulator genes in Human Periodontitis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
49,594 Da
NCBI Official Full Name
ZNF823 protein, partial
NCBI Official Synonym Full Names
zinc finger protein 823
NCBI Official Symbol
ZNF823
NCBI Official Synonym Symbols
HSZFP36
NCBI Protein Information
zinc finger protein 823
UniProt Protein Name
Zinc finger protein 823
Protein Family
UniProt Gene Name
ZNF823
UniProt Synonym Gene Names
ZFP36
UniProt Entry Name
ZN823_HUMAN
Uniprot Description
ZNF823: May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleus
Molecular Function: DNA binding; metal ion binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
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Product Notes
The ZNF823 znf823 (Catalog #AAA8102245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZNF823 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF823 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the ZNF823 znf823 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF823, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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