Rabbit E2F5 Polyclonal Antibody | anti-E2F5 antibody

Anti-E2F5 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102189

• Gene Names: E2F5; E2F-5
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: E2F5; Polyclonal Antibody; Anti-E2F5 Antibody; Rabbit Polyclonal; E2F5 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-E2F-5 Antibody; anti-E2F5 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human E2F5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-E2F5 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human E2F5
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human E2F5, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-E2F5 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:38 kDaObserved band size:30 kDa)

Immunoprecipitation (IP)

(E2F5 was immunoprecipitated using:Lane A:0.5 mg Raji Whole Cell Lysate1 uL anti-E2F5 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-E2F5 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 30 kDa)

Related Product Information for anti-E2F5 antibody

E2F transcription factors (E2F1 to 6) are central players in the control of animal cell proliferation as regulators of genes involved in cell cycle progression and transformation. E2F5 is commonly overexpressed in primary HCC and that E2F5 knockdown significantly repressed the growth of HCC cells. E2F Transcription Factor 5 Protein (E2F5) is considered to act primarily as a transcriptional repressor in the cell cycle. Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death worldwide, Forkhead box N3 (FOXN3) is an important member of the FOX family of transcription factors that plays an essential role in several cancers, FOXN3 repressed the mRNA and protein expression of E2F5, a reported potential oncogene, by inhibiting the promoter activity of E2F5. that FOXN3 functions as a tumor suppressor in HCC by downregulating the expression of E2F5.

NCBI and Uniprot Product Information

• NCBI GI #: 134142811
• NCBI GeneID: 1875
• NCBI Accession #: NP_001942.2
• NCBI GenBank Nucleotide #: NM_001951.3
• UniProt Accession #: Q15329; Q16601; Q92756; E9PBN9
• Molecular Weight: 20,192 Da
• NCBI Official Full Name: transcription factor E2F5 isoform 1
• NCBI Official Synonym Full Names: E2F transcription factor 5, p130-binding
• NCBI Official Symbol: E2F5
• NCBI Official Synonym Symbols: E2F-5
• NCBI Protein Information: transcription factor E2F5
• UniProt Protein Name: Transcription factor E2F5
• Protein Family: Transcription factor
• UniProt Gene Name: E2F5
• UniProt Synonym Gene Names: E2F-5
• UniProt Entry Name: E2F5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F5: a member of the E2F/DP family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4 than to other E2F/DP family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription initiation from RNA polymerase II promoter; organ morphogenesis; transcription, DNA-dependent; cell projection organization and biogenesis; regulation of cell cycle; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle