Immunoprecipitation (IP)
(GMFG was immunoprecipitated using:Lane A:0.5 mg MOLT-4 Whole Cell Lysate2 uL anti-GMFG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GMFG rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
Rabbit anti-Human GMFG Polyclonal Antibody | anti-GMFG antibody
Anti-GMFG Antibody, Rabbit Polyclonal
Gene Names
GMFG; GMF-GAMMA
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
GMFG; Polyclonal Antibody; Anti-GMFG Antibody; Rabbit Polyclonal; GMFG/glia maturation factor; gamma Antibody; Rabbit PAb; Antigen Affinity Purified; glia maturation factor; gamma; Anti-GMF-GAMMA Antibody; anti-GMFG antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GMFG/glia maturation factor, gamma
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GMFG antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IP: 1-2uL/mg of lysate
IP: 1-2uL/mg of lysate
Immunogen
Recombinant Human GMFG/glia maturation factor, gamma Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human GMFG/glia maturation factor, gamma (rh GMFG/glia maturation factor, gamma). GMFG/glia maturation factor, gamma specific IgG was purified by Human GMFG/glia maturation factor, gamma affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(GMFG was immunoprecipitated using:Lane A:0.5 mg MOLT-4 Whole Cell Lysate2 uL anti-GMFG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GMFG rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
Immunoprecipitation (IP)
(GMFG was immunoprecipitated using:Lane A:0.5 mg MOLT-4 Whole Cell Lysate2 uL anti-GMFG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GMFG rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)
Western Blot (WB)
(Anti-PAX8 rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:48 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PAX8 rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:48 kDa (We are unsure as to the identity of these extra bands.))
Related Product Information for anti-GMFG antibody
The glia maturation factor gamma (GMFG) is a cytokine-responsive protein in erythropoietin-induced and granulocyte-colony stimulating factor-induced hematopoietic lineage development. It may mediate the pluripotentiality and lineage commitment of human hematopoietic stem cells. GMFG expression independently predicts poorer prognosis in patients with EOC. Ectopic overexpression of GMFG contributes to the malignant biological behavior of ovarian cancer cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,801 Da
NCBI Official Full Name
glia maturation factor gamma isoform 2
NCBI Official Synonym Full Names
glia maturation factor, gamma
NCBI Official Symbol
GMFG
NCBI Official Synonym Symbols
GMF-GAMMA
NCBI Protein Information
glia maturation factor gamma
UniProt Protein Name
Glia maturation factor gamma
Protein Family
UniProt Gene Name
GMFG
UniProt Synonym Gene Names
GMF-gamma
UniProt Entry Name
GMFG_HUMAN
Uniprot Description
GMF-gamma: Belongs to the actin-binding proteins ADF family. GMF subfamily.
Protein type: Actin-binding
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: intracellular
Molecular Function: growth factor activity; protein kinase inhibitor activity; enzyme activator activity; actin binding
Biological Process: positive regulation of catalytic activity; negative regulation of protein kinase activity; protein amino acid phosphorylation
Research Articles on GMFG
Similar Products
Product Notes
The GMFG gmfg (Catalog #AAA8107588) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GMFG Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GMFG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the GMFG gmfg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GMFG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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