Western Blot (WB)
(Anti-TTF2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:130 kDaObserved band size:145 kDa)
Rabbit anti-Human TTF2 Polyclonal Antibody | anti-TTF2 antibody
Anti-TTF2 Antibody, Rabbit Polyclonal
IP: 1-2uL/mg of lysate
Western Blot (WB)
(Anti-TTF2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:130 kDaObserved band size:145 kDa)
Western Blot (WB)
(Anti-TTF2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:130 kDaObserved band size:145 kDa)
Immunoprecipitation (IP)
(TTF2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-TTF2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TTF2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 127 kDaObserved band size: 127 kDa)
Immunoprecipitation (IP)
(TTF2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-TTF2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TTF2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 127 kDaObserved band size: 127 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the SWI2/SNF2 family of proteins, which play a critical role in altering protein-DNA interactions. The encoded protein has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing. [provided by RefSeq, Jul 2008]
Uniprot Description
TTF2: DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Helicase; EC 3.6.4.-; Transcription regulation; EC 3.6.1.-; RNA splicing; DNA-binding
Chromosomal Location of Human Ortholog: 1p22
Cellular Component: spliceosome; transcription elongation factor complex; cytoplasm
Molecular Function: DNA-dependent ATPase activity; protein binding; DNA binding; zinc ion binding; ATP-dependent helicase activity; ATP binding
Biological Process: regulation of transcription, DNA-dependent; RNA splicing; mRNA processing; termination of RNA polymerase II transcription; transcription termination
Research Articles on TTF2
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Product Notes
The TTF2 ttf2 (Catalog #AAA8101267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TTF2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TTF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the TTF2 ttf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TTF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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