Rabbit anti-Mouse EMAP-II/AIMP1 Polyclonal Antibody | anti-AIMP1 antibody

Anti-EMAP-II/AIMP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8111933

• Gene Names: Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: EMAP-II/AIMP1; Polyclonal Antibody; Anti-EMAP-II/AIMP1 Antibody; Rabbit Polyclonal; AIMP1/EMAP II Antibody; Rabbit PAb; Antigen Affinity Purified; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; Anti-9830137A06Rik Antibody; Anti-AIMP1 Antibody; Anti-AIMP1/p43 Antibody; Anti-Emap2 Antibody; Anti-EMAPII Antibody; Anti-p43 Antibody; Anti-Scye1 Antibody; anti-AIMP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse AIMP1/EMAP II
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-AIMP1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Mouse AIMP1/EMAP II protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse AIMP1/EMAP II. AIMP1/EMAP II specific IgG was purified by Mouse AIMP1/EMAP II affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(Mouse SCYE1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-Mouse SCYE1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse SCYE1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)

Western Blot (WB)

(Anti-SCYE1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Rat brain Tissue LysateLane C: 293T Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-AIMP1 antibody

Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, also known as Multisynthase complex auxiliary component p43, Endothelial monocyte-activating polypeptide II, AIMP1, EMAP2 and SCYE1, is a nucleus protein which contains one tRNA-binding domain. AIMP1 (also known as p43) is a factor associated with a macromolecular aminoacyl-tRNA synthetase (ARS) complex but also plays diverse regulatory roles in various physiological processes. AIMP1 negatively regulates TGF-beta signaling via stabilization of Smurf2. It suggests the novel activity of AIMP1 as a component of negative feedback loop of TGF-beta signaling. Recently, it been demonstrated that AIMP1 is also secreted and acts as a novel pleiotropic cytokine. AIMP1 protein induces the maturation and activation of DCs, which skew the immune response toward a Th1 response. AIMP1 is known as a cytokine working in the control of angiogenesis, inflammation, and wound healing. AIMP1 is secreted from the pancreas upon glucose starvation, and it also plays a glucagon-like role in glucose homeostasis. Although AIMP1 was identified as a component of the macromolecular aminoacyl tRNA synthetase complex involved in the cellular translation process, it was also found to be secreted as a cytokine having complex physiological functions. Among these, AIMP1's angiostatic and immune stimulating activities suggest its potential use as a novel antitumor therapeutic protein. AIMP1 may exert its antitumor activity by inducing tumor-suppressing cytokines. Thus, AIMP1 may be useful as a novel anti-tumor agent.

References

Lee YS, et al. (2006) Antitumor activity of the novel human cytokine AIMP1 in an in vivo tumor model. Mol Cells. 21(2): 213-7.Park SG, et al. (2006) Hormonal activity of AIMP1/p43 for glucose homeostasis. Proc Natl Acad Sci U S A. 103(40): 14913-8.Kim E, et al. (2008) AIMP1/p43 protein induces the maturation of bone marrow-derived dendritic cells with T helper type 1-polarizing ability. J Immunol. 180(5): 2894-902.Lee YS, et al. (2008) AIMP1/p43 downregulates TGF-beta signaling via stabilization of smurf2. Biochem Biophys Res Commun. 371(3): 395-400.Han JM, et al. (2010) Antitumor activity and pharmacokinetic properties of ARS-interacting multi-functional protein 1 (AIMP1/p43). Cancer Lett. 287(2): 157-64.

NCBI and Uniprot Product Information

• NCBI GI #: 6754324
• NCBI GeneID: 16171
• NCBI Accession #: NP_034682.1
• NCBI GenBank Nucleotide #: NM_010552.3
• UniProt Accession #: Q62386; Q60971
• Molecular Weight: 17,490 Da
• NCBI Official Full Name: interleukin-17A
• NCBI Official Synonym Full Names: interleukin 17A
• NCBI Official Symbol: Il17a
• NCBI Official Synonym Symbols: Il17; Ctla8; IL-17; Ctla-8; IL-17A
• NCBI Protein Information: interleukin-17A; cytotoxic T-lymphocyte-associated antigen 8
• UniProt Protein Name: Interleukin-17A
• Protein Family: Aminoacyl tRNA synthase complex-interacting multifunctional protein
• UniProt Gene Name: Il17a
• UniProt Synonym Gene Names: Ctla8; Il17; IL-17; IL-17A; CTLA-8
• UniProt Entry Name: IL17_MOUSE

Uniprot Description

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function: cytokine activity

Biological Process: positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; positive regulation of transcription from RNA polymerase II promoter; inflammatory response