Rabbit anti-Human MDMX Polyclonal Antibody | anti-MDM4 antibody

Anti-MDMX Antibody, Rabbit Polyclonal

Cat. Num. AAA8107572

• Gene Names: MDM4; HDMX; MDMX; MRP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: MDMX; Polyclonal Antibody; Anti-MDMX Antibody; Rabbit Polyclonal; MDM4/MDMX Antibody; Rabbit PAb; Antigen Affinity Purified; MDM4; p53 regulator; Anti-HDMX Antibody; Anti-MRP1 Antibody; anti-MDM4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MDM4/MDMX
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MDM4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Human MDM4/MDMX Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human MDM4/MDMX (rh MDM4/MDMX). MDM4/MDMX specific IgG was purified by Human MDM4/MDMX affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MDM4 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:55 kDa Observed band size:68 kDa (We are unsure as to the identity of these extra bands.))

Related Product Information for anti-MDM4 antibody

MDM4 (MDM4 Regulator Of P53, also known as MDMX) is a Protein Coding gene. This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus and shows structural similarity to p53-binding protein MDM2. MDM4 is a promising target for cancer therapy, as it is undetectable in most normal adult tissues but often upregulated in cancer cells to dampen p53 tumor-suppressor function. MDM4, an essential negative regulator of the P53 tumor suppressor, is frequently overexpressed in cancer cells that harbor a wild-type P53. MDM4 is a key regulator of p53, whose biological activities depend on both transcriptional activity and transcription-independent mitochondrial functions. MDM4 binds to p53 and blocks its transcriptional activity.

NCBI and Uniprot Product Information

• NCBI GI #: 88702791
• NCBI GeneID: 4194
• NCBI Accession #: NP_002384.2
• NCBI GenBank Nucleotide #: NM_002393.4
• UniProt Accession #: O15151; Q2M2Y2; Q32SL2; Q6GS18; Q8IV83
• Molecular Weight: 49,541 Da
• NCBI Official Full Name: protein Mdm4 isoform 1
• NCBI Official Synonym Full Names: MDM4, p53 regulator
• NCBI Official Symbol: MDM4
• NCBI Official Synonym Symbols: HDMX; MDMX; MRP1
• NCBI Protein Information: protein Mdm4; protein Mdmx; MDM4 protein variant G; MDM4 protein variant Y; MDM4-related protein 1; mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog; double minute 4, human homolog of; p53-binding protein
• UniProt Protein Name: Protein Mdm4
• Protein Family: Protein
• UniProt Gene Name: MDM4
• UniProt Synonym Gene Names: MDMX
• UniProt Entry Name: MDM4_HUMAN

NCBI Description

This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

MDM4: Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with UPB2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53. Down-regulated by cisplatin. Expressed in all tissues tested with high levels in thymus. Belongs to the MDM2/MDM4 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; zinc ion binding

Biological Process: cell proliferation; negative regulation of cell proliferation; protein stabilization; DNA damage response, signal transduction by p53 class mediator; G0 to G1 transition; positive regulation of cell proliferation; protein complex assembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of protein catabolic process; negative regulation of apoptosis