Western Blot (WB)
(Anti-DPPA4 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human DPPA4 Polyclonal Antibody | anti-DPPA4 antibody
Anti-DPPA4 Antibody, Rabbit Polyclonal
Gene Names
DPPA4; 2410091M23Rik
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
DPPA4; Polyclonal Antibody; Anti-DPPA4 Antibody; Rabbit Polyclonal; DPPA4 Antibody; Rabbit PAb; Antigen Affinity Purified; developmental pluripotency associated 4; Anti-2410091M23Rik Antibody; anti-DPPA4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DPPA4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-DPPA4 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 4-8uL/mg of lysate
IP: 4-8uL/mg of lysate
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human DPPA4
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DPPA4, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-DPPA4 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-DPPA4 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(DPPA4 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate4 uL anti-DPPA4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DPPA4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 40 kDa)
Immunoprecipitation (IP)
(DPPA4 was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate4 uL anti-DPPA4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DPPA4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 40 kDa)
Related Product Information for anti-DPPA4 antibody
Developmental Pluripotency-Associated-4 (DPPA4) is one of the few core pluripotency genes lacking clearly defined molecular and cellular functions. DPPA4 is also mutated in some cancers. In direct transformation assays, we validated that DPPA4 is an oncogene in both mouse 3T3 cells and immortalized human dermal fibroblasts. Overexpression of DPPA4 generates oncogenic foci (sarcoma cells) and causes anchorage-independent growth. The in vitro transformed cells also give rise to tumors in immunodeficient mice. Developmental pluripotency associated 4 (DPPA4) is one of the uncharacterized genes that is highly expressed in embryonic stem (ES) cells. DPPA4 is associated with active chromatin and involved in the pluripotency of mouse ES cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,541 Da
NCBI Official Full Name
developmental pluripotency-associated protein 4
NCBI Official Synonym Full Names
developmental pluripotency associated 4
NCBI Official Symbol
DPPA4
NCBI Official Synonym Symbols
2410091M23Rik
NCBI Protein Information
developmental pluripotency-associated protein 4
UniProt Protein Name
Developmental pluripotency-associated protein 4
Protein Family
UniProt Gene Name
DPPA4
UniProt Entry Name
DPPA4_HUMAN
Uniprot Description
DPPA4: May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: 3q13.13
Cellular Component: nucleus
Molecular Function: protein binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Research Articles on DPPA4
Similar Products
Product Notes
The DPPA4 dppa4 (Catalog #AAA8100779) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DPPA4 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DPPA4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 4-8uL/mg of lysate. Researchers should empirically determine the suitability of the DPPA4 dppa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPPA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
