Rabbit anti-Human Latexin Polyclonal Antibody | anti-LXN antibody

Anti-Latexin Antibody, Rabbit Polyclonal

Cat. Num. AAA8101760

• Gene Names: LXN; ECI; TCI
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Latexin; Polyclonal Antibody; Anti-Latexin Antibody; Rabbit Polyclonal; Latexin/LXN/TCI Antibody; Rabbit PAb; Antigen Affinity Purified; latexin; Anti-ECI Antibody; Anti-TCI Antibody; anti-LXN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Latexin/LXN/TCI
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LXN antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human Latexin protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Latexin (rh Latexin). Latexin specific IgG was purified by human Latexin affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(LXN was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate2 uL anti-LXN rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LXN rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 25.7 kDaObserved band size: 25.7 kDa)

Western Blot (WB)

(Anti-LXN rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-LXN antibody

Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI, and LXN, is a cytoplasm protein that belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in the heart, prostate, ovary, kidney, pancreas, and colon. Latexin/LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in the human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including the brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. Also, Latexin is implicated in Alzheimer's disease.

References

Liu Q., et al., (2000), Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor. Mol. Biol. Rep. 27:241-246.Gauci S., et al., (2009), Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.nal. Chem. 81:4493-4501.Muzny D.M., et al., (2006), The DNA sequence, annotation and analysis of human chromosome 3.Nature 440:1194-1198.

NCBI and Uniprot Product Information

• NCBI GI #: 194473714
• NCBI GeneID: 56925
• NCBI Accession #: NP_064554.3
• NCBI GenBank Nucleotide #: NM_020169.3
• UniProt Accession #: Q9BS40; Q96PN2; Q9NQS6
• Molecular Weight: 25,750 Da
• NCBI Official Full Name: latexin
• NCBI Official Synonym Full Names: latexin
• NCBI Official Symbol: LXN
• NCBI Official Synonym Symbols: ECI; TCI
• NCBI Protein Information: latexin; MUM; endogenous carboxypeptidase inhibitor; tissue carboxypeptidase inhibitor
• UniProt Protein Name: Latexin
• Protein Family: Latexin
• UniProt Gene Name: LXN
• UniProt Synonym Gene Names: ECI; TCI
• UniProt Entry Name: LXN_HUMAN

NCBI Description

This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases. [provided by RefSeq, Oct 2008]

Uniprot Description

LXN: Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. Belongs to the protease inhibitor I47 (latexin) family.

Chromosomal Location of Human Ortholog: 3q25.32

Cellular Component: cytoplasm

Molecular Function: metalloendopeptidase inhibitor activity; heparin binding

Biological Process: detection of temperature stimulus involved in sensory perception of pain; inflammatory response