Rabbit anti-Human ATXN7L3 Polyclonal Antibody | anti-ATXN7L3 antibody

Anti-ATXN7L3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101977

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ATXN7L3; Polyclonal Antibody; Anti-ATXN7L3 Antibody; Rabbit Polyclonal; ATXN7L3 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-ATXN7L3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ATXN7L3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ATXN7L3 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 4-8uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human ATXN7L3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ATXN7L3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ATXN7L3 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Raji Whole Cell LysateLane C: K562 Whole Cell LysateLane D: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:39 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(ATXN7L3 was immunoprecipitated using:Lane A:0.5 mg Raji Whole Cell LysateLane B:0.5 mg K562 Whole Cell LysateLane C:0.5 mg THP-1 Whole Cell Lysate4 uL anti-ATXN7L3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ATXN7L3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 45 kDa)

Related Product Information for anti-ATXN7L3 antibody

ATXN7L3 contains a Sus1/ENY2-binding region in its N-terminal region, a ZnF-Sgf11 domain, and a SCA7 domain in its C-terminal region. The presence of ATXN7L3 is essential for the deubiquitinase activity of the DUB module. No stable complex could be formed in the absence of ATXN7L3. Moreover, the ZnF-Sgf11 domain of ATXN7L3 plays a pivotal role in the enzymatic activity, but is dispensable for the assembly, of the DUB module. The ZnF-Sgf11 domain of ATXN7L3 is essential for DUB activity toward H2Bub1 in vitro. The ZnF-Sgf11 domain is required for ATXN7L3 binding to nucleosomal DNA, and the crystal structure of the DUB module reveals that an arginine cluster in the ZnF-Sgf11 domain directly interacts with ubiquitinated nucleosomes and H2A/H2B heterodimer. Loss of ATXN7L3B, a paralog of ATXN7L3, may also be associated with neurodegenerative disease, as three family members exhibiting loss of chromosome region 12q21, where ATXN7L3B lies, exhibited motor and cognitive deficiencies, as well as learning difficulties and cerebellar ataxia. ATXN7L3B and ATXN7L3 share 74% identity within their N-terminal ~60 amino acid residues, including the Sus1/ENY2-binding region.

References

Li W, et al. (2016) Cytoplasmic atxn7l3b interferes with nuclear functions of the saga deubiquitinase module. Mol Cell Biol 36 (22): 2855-2866.Atanassov BS, et al. (2016) Atxn7l3 and eny2 coordinate activity of multiple h2b deubiquitinases important for cellular proliferation and tumor growth. Mol Cell 62 (4): 558-571.

NCBI and Uniprot Product Information

• NCBI GI #: 149588534
• NCBI GeneID: 56970
• NCBI Accession #: NP_001092303
• NCBI GenBank Nucleotide #: NM_001098833
• UniProt Accession #: Q14CW9; Q8IY68; Q96N40; Q9NPU5
• Molecular Weight: 39,599 Da
• NCBI Official Full Name: ataxin-7-like protein 3 isoform b
• NCBI Official Synonym Full Names: ataxin 7-like 3
• NCBI Official Symbol: ATXN7L3
• NCBI Protein Information: ataxin-7-like protein 3; ATXN7L3
• UniProt Protein Name: Ataxin-7-like protein 3
• Protein Family: Ataxin
• UniProt Gene Name: ATXN7L3
• UniProt Entry Name: AT7L3_HUMAN