Rabbit anti-Human HtrA2/Omi Polyclonal Antibody | anti-HTRA2 antibody

Anti-HtrA2/Omi Antibody, Rabbit Polyclonal

Cat. Num. AAA8103385

• Gene Names: HTRA2; OMI; PARK13; PRSS25
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: HtrA2/Omi; Polyclonal Antibody; Anti-HtrA2/Omi Antibody; Rabbit Polyclonal; HtrA2 Antibody; Rabbit PAb; Antigen Affinity Purified; HtrA serine peptidase 2; Anti-OMI Antibody; Anti-PARK13 Antibody; Anti-PRSS25 Antibody; anti-HTRA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human HtrA2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-HTRA2 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IP: 1-2uL/mg of lysate
• Immunogen: Recombinant Human HtrA2 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human HtrA2 (rh HtrA2). HtrA2 specific IgG was purified by Human HtrA2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(HTRA2 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HL-60 Whole Cell Lysate1 uL anti-HTRA2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HTRA2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 37 kDaObserved band size: 37 kDa)

Western Blot (WB)

(Anti-HTRA2 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLane C: HL60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:49 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-HTRA2 antibody

Serine protease HTRA2, also known as high-temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI and HTRA2, is a single-pass membrane protein that belongs to the peptidase S1B family. HTRA2 contains one PDZ (DHR) domain. HTRA2 is a serine protease that shows proteolytic activity against a non-specific substrate beta-casein. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity or by a BIRC inhibition-independent, caspase-independent, and serine protease activity-dependent mechanism. HTRA2 cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 of HTRA2 seems to be proteolytically inactive. Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) which is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity, and postural instability, as well as by a clinically significant response to treatment with levodopa.

Product Categories/Family for anti-HTRA2 antibody

Serine Proteases and Regulators

References

Faccio L., et al., 2000, J. Biol. Chem. 275:2581-2588. Gray C.W., et al., 2000, Eur. J. Biochem. 267:5699-5710. Suzuki Y., et al., 2001, Mol. Cell 8:613-621. Strauss K.M., et al., 2005, Hum. Mol. Genet. 14:2099-2111.

NCBI and Uniprot Product Information

• NCBI GI #: 7019477
• NCBI GeneID: 27429
• NCBI Accession #: NP_037379.1
• NCBI GenBank Nucleotide #: NM_013247.4
• UniProt Accession #: O43464; Q9HBZ4; Q9P0Y3; Q9P0Y4
• Molecular Weight: 48,841 Da
• NCBI Official Full Name: serine protease HTRA2, mitochondrial isoform 1 preproprotein
• NCBI Official Synonym Full Names: HtrA serine peptidase 2
• NCBI Official Symbol: HTRA2
• NCBI Official Synonym Symbols: OMI; PARK13; PRSS25
• NCBI Protein Information: serine protease HTRA2, mitochondrial; serine protease 25; protease, serine, 25; serine proteinase OMI; HtrA-like serine protease; Omi stress-regulated endoprotease; high temperature requirement protein A2
• UniProt Protein Name: Serine protease HTRA2, mitochondrial
• Protein Family: Serine protease
• UniProt Gene Name: HTRA2
• UniProt Synonym Gene Names: OMI; PRSS25; HtrA2
• UniProt Entry Name: HTRA2_HUMAN

NCBI Description

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

HTRA2: a serine proteinase that normally resides in mitochondrial membranes. It exists in two populations in mitochondria: as an unprocessed form probably attached to the inner mitochondrial membrane through a N-terminal transmembrane domain, and as a processed form containing a reaper motif at its N-terminus. Following apoptotic stimuli it is autoproteolytically activated and released into the cytosol, where it promotes programmed cell death in caspase-dependent and -independent manners. The amino-terminal reaper motif binds to the IAP (inhibitor of apoptosis) proteins cIAP1, cIAP2, and and XIAP, disrupting IAP-caspase complexes in a manner similar to Smac/DIABLO. Phosphorylation by the protein kinase Akt attenuates its serine protease and pro-apoptotic activities. The PDZ domain mediates interaction with Mxi2, an alternatively spliced form of the p38 stress-activated kinase. In contrast to its pro-apoptotic effects, targeted deletion in mice indicates that it is involved in protection against cell stress in striatial neurons. Defects in HTRA2 are the cause of Parkinson disease 13 (PARK13). Four alternatively spliced human isoforms have been described.

Protein type: Membrane protein, integral; EC 3.4.21.108; Mitochondrial; Protease

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: endoplasmic reticulum membrane; internal side of plasma membrane; cytoskeleton; membrane; mitochondrion; endoplasmic reticulum; mitochondrial membrane; mitochondrial intermembrane space; cytosol; nucleus; chromatin

Molecular Function: peptidase activity; protein binding; serine-type peptidase activity; serine-type endopeptidase activity; unfolded protein binding

Biological Process: mitochondrion organization and biogenesis; positive regulation of apoptosis; regulation of multicellular organism growth; positive regulation of caspase activity; response to herbicide; proteolysis; adult walking behavior; negative regulation of cell cycle; protein autoprocessing; DNA damage response, signal transduction resulting in induction of apoptosis; pentacyclic triterpenoid metabolic process; cellular protein catabolic process; forebrain development; neuron development; ceramide metabolic process

Disease: Parkinson Disease 13, Autosomal Dominant, Susceptibility To