Western Blot (WB)
(Anti-TRAM2 antibody, MBS176318, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Mouse TRAM2,39.0KD(162aa tag+R190-P370)Lane 1: Recombinant Mouse TRAM2 Protein 10ngLane 2: Recombinant Mouse TRAM2 Protein 5ngLane 3: Recombinant Mouse TRAM2 Protein 2.5ng)
Rabbit TRAM2 Polyclonal Antibody | anti-TRAM2 antibody
Anti-TRAM2 antibody
Gene Names
Tram2; C330003D03Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
TRAM2; Polyclonal Antibody; Anti-TRAM2 antibody; Translocating chain-associated membrane protein 2; translocation associated membrane protein 2; C330003D03Rik antibody; KIAA0057 antibody; MGC25725 antibody; OTTHUMP00000016607 antibody; TRAM like protein antibody; Tram2 antibody; TRAM2_HUMAN antibody; Translocating chain associating membrane protein 2 antibody; Translocating chain-associated membrane protein 2 antibody; Translocation associated membrane protein 2 antibody; anti-TRAM2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
370
Applicable Applications for anti-TRAM2 antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRAM2 (309-325aa RFIHSQLRHWREYWKEQ), identical to the related rat sequence, and different from the related human sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-TRAM2 antibody, MBS176318, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Mouse TRAM2,39.0KD(162aa tag+R190-P370)Lane 1: Recombinant Mouse TRAM2 Protein 10ngLane 2: Recombinant Mouse TRAM2 Protein 5ngLane 3: Recombinant Mouse TRAM2 Protein 2.5ng)
Western Blot (WB)
(Anti-TRAM2 antibody, MBS176318, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Mouse TRAM2,39.0KD(162aa tag+R190-P370)Lane 1: Recombinant Mouse TRAM2 Protein 10ngLane 2: Recombinant Mouse TRAM2 Protein 5ngLane 3: Recombinant Mouse TRAM2 Protein 2.5ng)
Related Product Information for anti-TRAM2 antibody
Description: Rabbit IgG polyclonal antibody for Translocating chain-associated membrane protein 2(TRAM2) detection. Tested with WB in Human, Mouse, Rat.
Background: TRAM2(Translocation-Associating Membrane Protein 2), also called KIAA0057, is a protein that in humans is encoded by the TRAM2 gene. TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum(ER) membrane. By genomic sequence analysis, Onuchic et al.(1999) mapped the TRAM2 gene to chromosome 6p21.1-p12 near the PKHD1 gene. Upon activation, quiescent hepatic stellate cells proliferate, change morphologically into myofibroblasts, and increase their synthesis of extracellular matrix proteins. Stefanovic et al.(2004) demonstrated that both TRAM2 and collagen type I are upregulated in activated rat and human hepatic stellate cells.
Background: TRAM2(Translocation-Associating Membrane Protein 2), also called KIAA0057, is a protein that in humans is encoded by the TRAM2 gene. TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum(ER) membrane. By genomic sequence analysis, Onuchic et al.(1999) mapped the TRAM2 gene to chromosome 6p21.1-p12 near the PKHD1 gene. Upon activation, quiescent hepatic stellate cells proliferate, change morphologically into myofibroblasts, and increase their synthesis of extracellular matrix proteins. Stefanovic et al.(2004) demonstrated that both TRAM2 and collagen type I are upregulated in activated rat and human hepatic stellate cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
43,183 Da
NCBI Official Full Name
Translocating chain-associated membrane protein 2
NCBI Official Synonym Full Names
translocating chain-associating membrane protein 2
NCBI Official Symbol
Tram2
NCBI Official Synonym Symbols
C330003D03Rik
NCBI Protein Information
translocating chain-associated membrane protein 2
UniProt Protein Name
Translocating chain-associated membrane protein 2
Protein Family
UniProt Gene Name
Tram2
UniProt Entry Name
TRAM2_MOUSE
Uniprot Description
TRAM2: Necessary for collagen type I synthesis. May couple the activity of the ER Ca(2+) pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca(2+) concentration at the site of collagen synthesis, and a high Ca(2+) concentration may be necessary for the function of molecular chaperones involved in collagen folding. Belongs to the TRAM family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Cellular Component: membrane; integral to membrane
Molecular Function: protein binding
Biological Process: protein transport; transport; collagen biosynthetic process
Research Articles on TRAM2
Similar Products
Product Notes
The TRAM2 tram2 (Catalog #AAA176318) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRAM2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRAM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TRAM2 tram2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
