Western Blot (WB)
(Anti-Ki67 antibody, MBS176064, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD(162aa tag+ K2967-I3256)Lane 1: Recombinant Human Ki67 Protein 10ngLane 2: Recombinant Human Ki67 Protein 5ngLane 3: Recombinant Human Ki67 Protein 2.5ng )
Rabbit anti-Human Ki67 Polyclonal Antibody | anti-MKI67 antibody
Anti-Ki67 antibody
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Ki67; Polyclonal Antibody; Anti-Ki67 antibody; Antigen KI-67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki 67 antibody; Antigen KI-67 antibody; Antigen KI67 antibody; KI67_HUMAN antibody; KIA antibody; MKI67 antibody; Proliferation related Ki 67 antigen antibody; RP11-380J17.2 antibody; anti-MKI67 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
3256
Applicable Applications for anti-MKI67 antibody
Western Blot (WB)
Application Notes
WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human
WB: the detection limit for MK167 is approximately 1ng/lane under reducing conditions.
Tested Species: In house tested species with positive results.
Predicted Species: Species Predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
WB: the detection limit for MK167 is approximately 1ng/lane under reducing conditions.
Tested Species: In house tested species with positive results.
Predicted Species: Species Predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Ki67 (3234-3256aa KKAEDNVCVKKIRTRSHRDSEDI).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Ki67 antibody, MBS176064, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD(162aa tag+ K2967-I3256)Lane 1: Recombinant Human Ki67 Protein 10ngLane 2: Recombinant Human Ki67 Protein 5ngLane 3: Recombinant Human Ki67 Protein 2.5ng )
Western Blot (WB)
(Anti-Ki67 antibody, MBS176064, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD(162aa tag+ K2967-I3256)Lane 1: Recombinant Human Ki67 Protein 10ngLane 2: Recombinant Human Ki67 Protein 5ngLane 3: Recombinant Human Ki67 Protein 2.5ng )
Related Product Information for anti-MKI67 antibody
Description: Rabbit IgG polyclonal antibody for Antigen KI-67(MKI67) detection. Tested with WB in Human.
Background: Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, Schonk et al.(1989) demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al.(1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al.(1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Background: Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, Schonk et al.(1989) demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al.(1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al.(1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Antigen KI-67
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
NCBI Protein Information
antigen KI-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; antigen identified by monoclonal antibody Ki-67
UniProt Protein Name
Antigen KI-67
Protein Family
UniProt Gene Name
MKI67
UniProt Entry Name
KI67_HUMAN
NCBI Description
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Uniprot Description
KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.
Protein type: Cell cycle regulation; Nucleolus
Chromosomal Location of Human Ortholog: 10q26.2
Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region
Molecular Function: protein C-terminus binding; protein binding; ATP binding
Biological Process: cell proliferation; meiosis
Research Articles on MKI67
Similar Products
Product Notes
The MKI67 mki67 (Catalog #AAA176064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ki67 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ki67 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human WB: the detection limit for MK167 is approximately 1ng/lane under reducing conditions. Tested Species: In house tested species with positive results. Predicted Species: Species Predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the MKI67 mki67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ki67, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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