Rabbit B MyB Polyclonal Antibody | anti-MYBL2 antibody

Anti-B MyB antibody

Cat. Num. AAA176151

• Gene Names: MYBL2; BMYB; B-MYB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: B MyB; Polyclonal Antibody; Anti-B MyB antibody; Myb-related protein B; v-myb myeloblastosis viral oncogene homolog(avian)-like 2; B-Myb antibody; BMyB antibody; MGC15600 antibody; MYB L2 antibody; Myb related protein B antibody; Myb-like protein 2 antibody; Myb-related protein B antibody; MybB antibody; MYBB_HUMAN antibody; MYBL 2 antibody; MYBL2 antibody; v myb avian myeloblastosis viral oncogene homolog like 2 antibody; v myb myeloblastosis viral oncogene homolog(avian) like 2 antibody; v myb myeloblastosis viral oncogene homolog like 2 antibody; anti-MYBL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 700

• Applicable Applications for anti-MYBL2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human B MyB (1-17aa MSRRTRCEDLDELHYQD), identical to the related mouse sequence and different from the related rat sequence by two amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-B MyB antibody, MBS176151, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human MYBL2, 46.8KD(162aa tag+ M1-L245)Lane 1: Recombinant Human MYBL2 Protein 10ngLane 2: Recombinant Human MYBL2 Protein 5ngLane 3: Recombinant Human MYBL2 Protein 2.5ng )

Immunohistochemistry (IHC)

(Anti-B MyB antibody, MBS176151, IHC(P)IHC(P): Rat Brain Tissue )

Western Blot (WB)

(Anti-B MyB antibody, MBS176151, Western blottingLane 1: Rat Spleen Tissue LysateLane 2: Rat Thymus Tissue LysateLane 3: Rat Brain Tissue LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: MCF-7 Cell Lysate )

Immunocytochemistry (ICC)

(Anti-B MyB antibody, MBS176151, ICCICC: A549 Cell )

Immunohistochemistry (IHC)

(Anti-B MyB antibody, MBS176151, IHC(P)IHC(P): Human Tonsil Tissue )

Related Product Information for anti-MYBL2 antibody

Description: Rabbit IgG polyclonal antibody for Myb-related protein B(MYBL2) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: MYBL2(V-MYB avian myeloblastosis viral oncogene homolog-like 2), also called MYB-RELATED GENE BMYB, is a protein that in humans is encoded by the MYBL2 gene. The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. Barletta et al.(1991) assigned the MYBL2 gene to chromosome Xq13. However, Noben-Trauth et al.(1996) demonstrated that this assignment was an error. Using mouse Mybl2 cDNA clones as probes, they assigned Mybl2 in an interspecific backcross panel to distal mouse chromosome 2. Using human cDNA probes in combination with fluorescence in situ hybridization analysis, they localized MYBL2 to chromosome 20q13.1, a region that is commonly deleted in myeloid disorders and shows high homology of synteny to mouse chromosome 2. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 127584
• NCBI GeneID: 4605
• UniProt Accession #: P10244; Q53F07; B2RBS5; B7Z8D9; F8W6N6
• Molecular Weight: 75,865 Da
• NCBI Official Full Name: Myb-related protein B
• NCBI Official Synonym Full Names: v-myb avian myeloblastosis viral oncogene homolog-like 2
• NCBI Official Symbol: MYBL2
• NCBI Official Synonym Symbols: BMYB; B-MYB
• NCBI Protein Information: myb-related protein B; myb-like protein 2; v-myb myeloblastosis viral oncogene homolog-like 2
• UniProt Protein Name: Myb-related protein B
• Protein Family: Myb-related protein
• UniProt Gene Name: MYBL2
• UniProt Synonym Gene Names: BMYB; B-Myb
• UniProt Entry Name: MYBB_HUMAN

NCBI Description

The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

B-Myb: a transcription factor involved in cell cycle progression. Phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm

Molecular Function: protein binding; chromatin binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of cell cycle; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; G2/M transition of mitotic cell cycle