Rabbit anti-Human DLL3 Polyclonal Antibody | anti-DLL3 antibody

Anti-DLL3 antibody

Cat. Num. AAA176310

• Gene Names: DLL3; SCDO1
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: DLL3; Polyclonal Antibody; Anti-DLL3 antibody; Delta-like protein 3; delta-like 3(Drosophila); Delta Drosophila like 3 antibody; Delta like 3 Drosophila antibody; Delta like 3 homolog Drosophila antibody; Delta like 3 protein antibody; Delta like protein 3 precursor antibody; Delta-like protein 3 antibody; Delta3 antibody; Dll3 antibody; DLL3_HUMAN antibody; Drosophila Delta homolog 3 antibody; SCDO1 antibody; SCOD1 antibody; Spondylocostal dysostosis autosomal recessive antibody; anti-DLL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 618

• Applicable Applications for anti-DLL3 antibody: Western Blot (WB)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DLL3 (599-618aa RAGQRQHLLFPYPSSILSVK).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-DLL3 antibody, MBS176310, Western blottingAll lanes: Anti DLL3 (MBS176310) at 0.5ug/mlLane 1: Recombinant Human DLL3 Protein 10ngLane 2: Recombinant Human DLL3 Protein 5ngLane 3: Recombinant Human DLL3 Protein 2.5ngLane 4: Recombinant Human DLL3 Protein 1.25ngPredicted bind size: 41KDObserved bind size: 41KD )

Related Product Information for anti-DLL3 antibody

Description: Rabbit IgG polyclonal antibody for Delta-like protein 3(DLL3) detection. Tested with WB in Human.
Background: DLL3(DELTA-LIKE 3) also known as DELTA, DROSOPHILA, HOMOLOG OF, is a protein which in humans is encoded by the DLL3 gene. This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutation in the mouse delta-like 3 gene(Dll3), which is homologous to the Notch-ligand delta in Drosophila, results in the mouse 'pudgy' phenotype. The human DLL3 gene was identified within a critical interval, mapped in 2 consanguineous Arab-Israeli and Pakistani SCDO1 pedigrees, of 7.8 cM at 19q13.1-q13.3 between D19S570 and D19S908(Bulman et al., 2000). Dunwoodie et al.(1997) presented results suggesting that mouse Dll3 may complement the function of other delta homologs during early pattern formation in the mouse embryo. In humans, the fact that mutations in genes required for oscillation, such as DLL3, result in abnormal segmentation of the vertebral column suggests that the segmentation clock also acts during human embryonic development. This residue is highly conserved in Delta proteins from Drosophila to humans, and the substitution of a charged polar for a nonpolar residue may disrupt the conformation of the DLL3 protein.

NCBI and Uniprot Product Information

• NCBI GI #: 12229810
• NCBI GeneID: 10683
• UniProt Accession #: Q9NYJ7; Q8NBS4; E9PFG2
• Molecular Weight: 61,178 Da
• NCBI Official Full Name: Delta-like protein 3
• NCBI Official Synonym Full Names: delta-like 3 (Drosophila)
• NCBI Official Symbol: DLL3
• NCBI Official Synonym Symbols: SCDO1
• NCBI Protein Information: delta-like protein 3; delta3; drosophila Delta homolog 3
• UniProt Protein Name: Delta-like protein 3
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL3
• UniProt Synonym Gene Names: Delta3
• UniProt Entry Name: DLL3_HUMAN

NCBI Description

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL3: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Can bind and activate Notch-1 or another Notch receptor.

Protein type: Membrane protein, integral; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: integral to membrane

Molecular Function: Notch binding

Biological Process: paraxial mesoderm development; compartment specification; somitogenesis; Notch signaling pathway; skeletal development; negative regulation of neurogenesis

Disease: Spondylocostal Dysostosis 1, Autosomal Recessive