Mouse anti-Human CD13 Monoclonal Antibody | anti-CD13 antibody

anti-human CD13-Biotin

Cat. Num. AAA666330

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD13; Monoclonal Antibody; anti-human CD13-Biotin; Monoclonal anti-human CD13/Biotin; anti-CD13 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2ak
• Clone Number: 22A5
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-CD13 antibody: Flow Cytometry (FC/FACS), FPS
• Performance: Five x 10^5 cultured THP-1 cells were washed and pre incubated ~5 minutes with 20ul of 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD13/Biotin at 10 ug/ml. Cells were washed twice and incubated with 50 ul of 2 degree reagent Streptaviden/R-PE (MBS666783) after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.19 log₁₀ fluorescent units when compared to a Mouse IgG2a/Biotin negative control (MBS666441) at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD13 antibody (MBS666228).
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human osteosarcoma tissue
• Preparation and Storage: Store at 2 - 5°C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD13 antibody

Information: Human CD13 is a zinc-binding aminopeptidase-N enzyme expressed on the surface of myeloid cells. IL-4 will upregulate expression of CD13 which may play an anti-inflammatory role. Antibody 22A5 recognizes the cell surface aminopeptidase-N enzyme.

References

M.A. Horton, et al, (1985) Cancer Res 45: 5663-5669. R.A. Ashmun & A.T. Look (1990) Blood 75: 462-471. P.T.W. van Hal, et al, (1994) J Immunol 153: 2718-2728.