Western Blot (WB)
(Anti-TRAF2 antibody, MBS176268, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human TRAF2, 30.4KD(162aa tag+ Q261-V369)Lane 1: Recombinant Human TRAF2 Protein 10ngLane 2: Recombinant Human TRAF2 Protein 5ngLane 3: Recombinant Human TRAF2 Protein 2.5ng)
Rabbit TRAF2 Polyclonal Antibody | anti-TRAF2 antibody
Anti-TRAF2 antibody
Western Blot (WB)
(Anti-TRAF2 antibody, MBS176268, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human TRAF2, 30.4KD(162aa tag+ Q261-V369)Lane 1: Recombinant Human TRAF2 Protein 10ngLane 2: Recombinant Human TRAF2 Protein 5ngLane 3: Recombinant Human TRAF2 Protein 2.5ng)
Western Blot (WB)
(Anti-TRAF2 antibody, MBS176268, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human TRAF2, 30.4KD(162aa tag+ Q261-V369)Lane 1: Recombinant Human TRAF2 Protein 10ngLane 2: Recombinant Human TRAF2 Protein 5ngLane 3: Recombinant Human TRAF2 Protein 2.5ng)
Immunohistochemistry (IHC)
(Anti-TRAF2 antibody, MBS176268, IHC(P)IHC(P): Human Tonsil Tissue )
Immunohistochemistry (IHC)
(Anti-TRAF2 antibody, MBS176268, IHC(P)IHC(P): Human Tonsil Tissue )
Immunocytochemistry (ICC)
(Anti-TRAF2 antibody, MBS176268, ICCICC: HELA Cell )
Immunocytochemistry (ICC)
(Anti-TRAF2 antibody, MBS176268, ICCICC: HELA Cell )
Background: TRAF2(TNF Receptor-Associated Factor 2), also called TRAP, is a protein that in humans is encoded by the TRAF2 gene. The protein encoded by this gene is a member of the TNF receptor(TNFR) associated factor(TRAF) protein family. TRAF2 is a common signal transducer for TNFR2 and CD40 that mediates activation of NF-kappa-B. Rothe et al.(1996) identified ITRAF, which binds to TRAF1, TRAF2, and TRAF3, and that when overexpressed inhibits TRAF2-mediated NF-kappa-B activation. They proposed that ITRAF is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Kanamori et al.(2002) found that mouse Traf2 interacted directly with T2bp, and they presented evidence that T2BP is involved in TNF-mediated signaling by its interaction with TRAF2.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
TRAF2: Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Homotrimer, and heterotrimer with TRAF1 and TRAF3 (via TRAF domain). The domain containing the RING-type and the first TRAF-type zinc finger can also form homodimers (in vitro). Interacts with TNFRSF1B/TNFR2, TNFRSF4, TNFRSF5/CD40, CD27/TNFRSF7, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13B/TACI, TNFRSF14, TNFRSF16/NGFR, TNFRSF17/BCMA, TNFRSF18/AITR, TNFRSF19/TROY, TNFRSF19L/RELT, XEDAR, EDAR, Epstein-Barr virus BNFL1/LMP-1 and IL15RA. Interacts with CDK9, CSK, MAP3K1, MAP3K5, MAP3K11, MAP3K14, MAP4K2, RIPK1, RIPK2, TNIK, TBK1, SPHK1, TRADD, TRAFD1, TRAIP, TANK/ITRAF, TNFAIP3, TDP2, MAVS/IPS1, TICAM1 and TRPC4AP. Interacts with CASP8AP2, NFATC2IP, PEG3 and HIVEP3. Interacts with BIRC2 and BIRC3 N- terminus; a single BIRC2 or BIRC3 molecule interacts with a heterotrimer formed by TRAF1 and TRAF2, or a TRAF2 homotrimer. Identified in a complex composed of TRAF2, TRAF3, BIRC2 and BIRC3. Interaction with BIRC2 and/or BIRC3 is essential for degradation of NFKBIA and activation of NF-kappa-B. Interacts with CYLD, USP48, DAB2IP, IKKA and IKKB. Identified in a complex with TNFRSF1A, RIPK1 and IKKB. Interacts (via 'Lys-63'-linked polyubiquitin chains) with TAB2 and TAB3. Interacts with ERN1 and TAOK3. Interaction with TAOK3 is facilitated under ER stress conditions, such as treatment with tunicamycin, and may promote TRAF2 phosphorylation. Has very low E3 ubiquitin ligase activity in the absence of sphingosine-1-phosphate. E3 ubiquitin ligase activity is strongly activated by cytoplasmic sphingosine-1- phosphate. Belongs to the TNF receptor-associated factor family. A subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; Autophagy; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: internal side of plasma membrane; cytoplasm; vesicle membrane; cell cortex; cytosol; ubiquitin ligase complex; lipid raft
Molecular Function: sphingolipid binding; identical protein binding; zinc ion binding; ubiquitin-protein ligase activity; CD40 receptor binding; mitogen-activated protein kinase kinase kinase binding; protein phosphatase binding; tumor necrosis factor receptor binding; protein binding; signal transducer activity; enzyme binding; thioesterase binding; ubiquitin protein ligase binding; protein complex binding; ligase activity
Biological Process: caspase activation; positive regulation of JNK activity; protein autoubiquitination; apoptosis; protein heterooligomerization; activation of NF-kappaB-inducing kinase; positive regulation of T cell cytokine production; signal transduction; activation of NF-kappaB transcription factor; regulation of apoptosis; cellular protein complex assembly; tumor necrosis factor-mediated signaling pathway; positive regulation of interleukin-2 production; innate immune response; positive regulation of transcription factor activity; protein complex assembly; protein catabolic process; positive regulation of T cell activation; regulation of immunoglobulin secretion
Research Articles on TRAF2
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Product Notes
The TRAF2 traf2 (Catalog #AAA176268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRAF2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRAF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the TRAF2 traf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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