Rabbit anti-Rat GABAA Receptor, g 2 (Ser327) Polyclonal Antibody | anti-GABRG2 antibody

Anti-Phospho-Ser327 GABAA Receptor, gamma2 subunit

Cat. Num. AAA502056

• Reactivity: Rat
• Applications: Dot Blot, Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Synonyms: GABAA Receptor; g 2 (Ser327); Polyclonal Antibody; Anti-Phospho-Ser327 GABAA Receptor; gamma2 subunit; anti-GABRG2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for ~45k GABAA receptor gamma y2 subunit phosphorylated at Ser327 in Western Blots of rat brain homogenate.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 466

• Applicable Applications for anti-GABRG2 antibody: Dot Blots (DB), Western Blot (WB)
• Application Notes: Quality Control: ; DB: 1:1000; WB: 1:1000; ; Quality Control Tests: Western Blots performed on each lot.
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser327 of the GABAA receptor, gamma2 subunit.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 conjugated to KLH
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, mouse, human and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. It has recently been suggested that PKCepsilon regulates the sensitivity of GABAA alpha1beta2gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2 (Qi et al., 2007)
• Preparation and Storage: For long term storage ~80 degree C is recommended, but shorter term storage at ~20 degree C is also acceptable as aliquots maybe taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 degree C.

Testing Data

Related Product Information for anti-GABRG2 antibody

Affinity purified rabbit polyclonal antibody

References

• Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.
• Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653
• Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629.
• Qi ZH, Song M, Wallace MJ, Wang D, Newton PM, McMahon T, Chou WH, Zhang C, Shokat KM, Messing RO (2007) Protein kinase Cepsilon regulates gamma-aminobutyrate type A receptor sensitivity to ethanol and benzodiazepines through phosphorylation of gamma2 subunits. J Biol Chem 282(45):33052-63.

NCBI and Uniprot Product Information

• NCBI GI #: 34328527
• NCBI GeneID: 29709
• NCBI Accession #: NP_899156.1
• NCBI GenBank Nucleotide #: NM_183327.1
• UniProt Accession #: P18508
• Molecular Weight: 45
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit gamma-2
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, gamma 2
• NCBI Official Symbol: Gabrg2
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit gamma-2; GABA(A) receptor subunit gamma-2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit gamma-2
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: Gabrg2
• UniProt Entry Name: GBRG2_RAT

NCBI Description

subunit of GABA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain [RGD, Feb 2006]

Uniprot Description

Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Post-translational modification: Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability

By similarity.

Miscellaneous: This subunit carries the benzodiazepine binding site.

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. [View classification]