Rabbit anti-Rat GABAB Receptor (Ser783), R2-Subunit Polyclonal Antibody | anti-GABBR2 antibody

Anti-Phospho-Ser783 GABAB R2

Cat. Num. AAA502057

• Gene Names: Gabbr2; Gpr51
• Reactivity: Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Synonyms: GABAB Receptor (Ser783); R2-Subunit; Polyclonal Antibody; Anti-Phospho-Ser783 GABAB R2; anti-GABBR2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for ~102k GABAB R2 phosphorylated at Ser783. Immunolabeling of the GABAB R2 band is completely blocked by lambda-phosphatase treatment.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 940

• Applicable Applications for anti-GABBR2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IF: 1:500 (Kuramoto et al., 2007)
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser783 of GABAB R2.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primates
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, non-human primate, chicken, bovine and Xenopus tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-GABBR2 antibody

Affinity purified rabbit polyclonal antibody

References

• Kuramoto N, Wilkins ME, Fairfax BP, Revilla-Sanchez R, Terunuma M, Tamaki K, Iemata M, Warren N, Couve A, Calver A, Horvath Z, Freeman K, Carling D, Huang L, Gonzales C, Cooper E, Smart TG, Pangalos MN, Moss SJ (2007) Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron 53:233-247.

NCBI and Uniprot Product Information

• NCBI GI #: 13929138
• NCBI GeneID: 83633
• NCBI Accession #: NP_113990.1
• NCBI GenBank Nucleotide #: NM_031802.1
• UniProt Accession #: O88871; Q9JK36; Q9QWU2
• Molecular Weight: 102
• NCBI Official Full Name: gamma-aminobutyric acid type B receptor subunit 2
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) B receptor 2
• NCBI Official Symbol: Gabbr2
• NCBI Official Synonym Symbols: Gpr51
• NCBI Protein Information: gamma-aminobutyric acid type B receptor subunit 2; gb2; GABABR2; GABA-BR2; GABA-B-R2; GABA-B receptor 2; GABA-B R2 receptor; G protein-coupled receptor 51; G-protein coupled receptor 51
• UniProt Protein Name: Gamma-aminobutyric acid type B receptor subunit 2
• Protein Family: Gamma-aminobutyric acid type B receptor
• UniProt Gene Name: Gabbr2
• UniProt Synonym Gene Names: Gpr51; GABA-B receptor 2; GABA-B-R2; GABA-BR2; GABABR2; Gb2
• UniProt Entry Name: GABR2_RAT

NCBI Description

combines with GABA-B1 to form functional GABA-B receptors; inhibits high voltage activated calcium ion channels [RGD, Feb 2006]

Uniprot Description

Function: Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Ref.2

Subunit structure: Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts with ATF4 via its C-terminal region. Ref.2 Ref.5 Ref.6

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note: Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane. Ref.2

Tissue specificity: Expressed in brain cortex, hippocampus, medial habenula, thalamus and cerebellum. Coexpression is seen in cerebellum. Ref.2 Ref.3

Developmental stage: Abundant in brain cortex and cerebellum throughout postnatal development whereas its expression in spinal cord gradually decreases.

Domain: Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.

Sequence similarities: Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.