Rabbit anti-Rat GABAA Receptor g2 Polyclonal Antibody | anti-GABRG2 antibody

Anti-GABAA Receptor, gamma2-Subunit

Cat. Num. AAA502054

• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Neat Serum (Unpurified neat serum.)
• Synonyms: GABAA Receptor g2; Polyclonal Antibody; Anti-GABAA Receptor; gamma2-Subunit; anti-GABRG2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~46k gamma2-subunit of the GABAA receptor in Western blots of rat brain extracts.
• Purity/Purification: Neat Serum (Unpurified neat serum.)
• Form/Format: 50 ul neat serum. Adequate material to conduct 10 mini-Western blots.
• Sequence Length: 466

• Applicable Applications for anti-GABRG2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: 1:400
• Antigen: Peptide representing a sequence that is specific for the gamma2-subunit of rat GABAA receptor. The sequence of the immunogen has no homology with any other GABAA R-subunit.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues specific to the gamma 2 subunit conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Chicken, human, mouse, non-human primates and zebra fish
• Species Reactivity Note: The antibody has been directly tested for reactivity in rat tissue. It is anticipated that the antibody will also work with bovine, chicken, human, mouse, non-human primate and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-GABRG2 antibody

Rabbit polyclonal antibody

References

• Brandon NJ, Jovanovic JN, Colledge M, Kittler JT, Brandon JM, Scott JD, Moss SJ (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor beta-subunits. Mol Cell Neurosci 22:87-97
• McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592.
• Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
• Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629.
• Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.

NCBI and Uniprot Product Information

• NCBI GI #: 34328527
• NCBI GeneID: 29709
• NCBI Accession #: NP_899156.1
• NCBI GenBank Nucleotide #: NM_183327.1
• UniProt Accession #: P18508
• Molecular Weight: 46
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit gamma-2
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, gamma 2
• NCBI Official Symbol: Gabrg2
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit gamma-2; GABA(A) receptor subunit gamma-2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit gamma-2
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: Gabrg2
• UniProt Entry Name: GBRG2_RAT

NCBI Description

subunit of GABA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain [RGD, Feb 2006]

Uniprot Description

Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Post-translational modification: Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability

By similarity.

Miscellaneous: This subunit carries the benzodiazepine binding site.

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. [View classification]