Rabbit anti-Rat, mouse Metabotropic Glutamate Receptor 7 (Ser862) Polyclonal Antibody | anti-GRM7 antibody

Anti-Phospho Ser862 mGluR7

Cat. Num. AAA502167

• Reactivity: Rat, mouse
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephospho-peptide columns.)
• Synonyms: Metabotropic Glutamate Receptor 7 (Ser862); Polyclonal Antibody; Anti-Phospho Ser862 mGluR7; anti-GRM7 antibody

• Host: Rabbit
• Reactivity: Rat, mouse
• Clonality: Polyclonal
• Specificity: Specific for the ~102k mGluR7 protein phosphorylated at Ser862. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephospho-peptide columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 915

• Applicable Applications for anti-GRM7 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding phospho-Ser862 of rat mGluR7.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser862 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human, non-human primate and zebrafish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in mouse and rat tissues. It is anticipated that the antibody will also work with bovine, canine, human, non-human primate and zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). The mGluRs are divided into three groups based on sequence identity and pharmacological properties: group I (mGluR1 and mGluR5) are localized in the perisynaptic region of the postsynaptic membrane, whereas group II (mGlur2 and mGluR3) and group III (mGluR4,6,7 and 8) are localized predominantly at presynaptic terminals. PKC phosphorylation of serine 862 on mGluR7 has been shown to be critical for stabilizing receptor surface expression and promoting binding to the synaptic PDZ-domain-containing protein PICK1 (Suh et al., 2008).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-GRM7 antibody

Affinity purified rabbit polyclonal antibody

References

• Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SSG (2004) Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis. J Neurosci 24:8752-8761.
• Francesconi W, Cammalleri M, Sanna PP (2004) The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region. Brain Res 1022:12-18.
• Wilson RI, Nicoll RA (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature (London) 410:588-592.
• Suh YH, Pelkey KA, Lavezzari G, Roche PA, Huganir RL, McBain CJ, Roche KW. (2008) Corequirement of PICK1 binding and PKC phosphorylation for stable surface expression of the metabotropic glutamate receptor mGluR7. Neuron. 58(5):736-48

NCBI and Uniprot Product Information

• NCBI GI #: 13591963
• NCBI GeneID: 81672
• NCBI Accession #: NP_112302.1
• NCBI GenBank Nucleotide #: NM_031040.1
• UniProt Accession #: P35400
• Molecular Weight: 102
• NCBI Official Full Name: metabotropic glutamate receptor 7
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 7
• NCBI Official Symbol: Grm7
• NCBI Protein Information: metabotropic glutamate receptor 7; mGluR7; metabotropic glutamate receptor mGluR7; metabotropic glutamate receptor subtype 7b
• UniProt Protein Name: Metabotropic glutamate receptor 7
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: Grm7
• UniProt Synonym Gene Names: Gprc1g; Mglur7; mGluR7
• UniProt Entry Name: GRM7_RAT

NCBI Description

G-protein coupled receptor that inhibits glutamate release; acts via cAMP inhibitory and Ca+2 channel inhibitory pathways [RGD, Feb 2006]

Uniprot Description

Function: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Ref.1 Ref.2

Subunit structure: Interacts with PICK1. Ref.3

Subcellular location: Cell membrane; Multi-pass membrane protein.

Tissue specificity: Widely distributed throughout the brain. Ref.1 Ref.2

Sequence similarities: Belongs to the G-protein coupled receptor 3 family.