Rabbit anti-Rat Synaptotagmin (Ser309) Polyclonal Antibody | anti-SYT1 antibody

Anti-Phospho-Ser309 Synaptotagmin

Cat. Num. AAA502122

• Gene Names: Syt1; P65
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Synaptotagmin (Ser309); Polyclonal Antibody; Anti-Phospho-Ser309 Synaptotagmin; anti-SYT1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~60k-62k synaptotagmin protein phosphorylated at Ser309.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 421

• Applicable Applications for anti-SYT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: 1:400
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser309 of synaptotagmin.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primate and zebra fish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-SYT1 antibody

Affinity purified rabbit polyclonal antibody

References

• Fernandez-Chacon R, Konigstorfer A, Gerber SH, Garcia J, Matos MF, Stevens CF, Brose N, Rizo J, Rosenmund C, Sudhof TC (2001) Synaptotagmin I functions as a calcium regulator of release probability. Nature (London) 410:41-49.
• Hilfiker S, Pieribone VA, Nordstedt C, Greengard P, Czernik AJ (1999) Regulation of synaptotagmin I phosphorylation by multiple protein kinases. J Neurochem 73:921-932.
• Lee BH, Min X, Heise CJ, Xu BE, Chen S, Shu H, Luby-Phelps K, Goldsmith EJ, Cobb MH (2004) WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Mol Cell 15:741-751.
• Poskanzer KE, Marek KW, Sweeney ST, Davis GW (2003) Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature (London) 426:559-563.
• Wang CT, Lu JC, Bai JH, Chang PY, Martin TFJ, Chapman ER, Jackson MB (2003) Different domains of synaptotagmin control the choice between kiss-and-run and full fusion. Nature (London) 424:943-947.

NCBI and Uniprot Product Information

• NCBI GI #: 148356226
• NCBI GeneID: 25716
• NCBI Accession #: NP_001028852.2
• NCBI GenBank Nucleotide #: NM_001033680.2
• UniProt Accession #: P21707; Q3S2E6; Q707P5
• Molecular Weight: 60/62
• NCBI Official Full Name: synaptotagmin-1
• NCBI Official Synonym Full Names: synaptotagmin I
• NCBI Official Symbol: Syt1
• NCBI Official Synonym Symbols: P65
• NCBI Protein Information: synaptotagmin-1; sytI; synaptotagmin 1
• UniProt Protein Name: Synaptotagmin-1
• Protein Family: Synaptotagmin
• UniProt Gene Name: Syt1
• UniProt Synonym Gene Names: SytI
• UniProt Entry Name: SYT1_RAT

NCBI Description

integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006]

Uniprot Description

Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2.

Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.

Subunit structure: Homotetramer

Probable. Interacts with SCAMP5 and STON2. Forms a complex with SV2B, syntaxin 1 and SNAP25

By similarity. Interacts with RIMS1. Interacts with SV2A, SV2B and SV2C. Ref.5 Ref.6 Ref.8

Subcellular location: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass membrane protein. Cytoplasm. Note: Synaptic vesicles and chromaffin granules.

Tissue specificity: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues.

Domain: The first C2 domain mediates Ca2+-dependent phospholipid binding. Ref.5The second C2 domain mediates interaction with SV2A and probably with STN2. Ref.5

Sequence similarities: Belongs to the synaptotagmin family.Contains 2 C2 domains.