Rabbit anti-Rat, mouse GABAA Receptor a 6 Polyclonal Antibody | anti-GABRA6 antibody

Anti-GABAA Receptor, alpha6-Subunit

Cat. Num. AAA502049

• Reactivity: Rat, mouse
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)
• Synonyms: GABAA Receptor a 6; Polyclonal Antibody; Anti-GABAA Receptor; alpha6-Subunit; anti-GABRA6 antibody

• Host: Rabbit
• Reactivity: Rat, mouse
• Clonality: Polyclonal
• Specificity: Specific for the ~57k alpha6-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha6-subunit knockout animals.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 453

• Applicable Applications for anti-GABRA6 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Fusion protein from the cytoplasmic loop of the alpha6-subunit of rat GABAA receptor.
• Immunogen Information: Fusion protein from the cytoplasmic loop of the alpha 6 subunit
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat and mouse tissue.
• Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-GABRA6 antibody

Affinity purified rabbit polyclonal antibody

References

• McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592.
• Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
• Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629.
• Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480
• Pöltl A, Hauer B, Fuchs K, Tretter V, Sieghart W (2003) Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455

NCBI and Uniprot Product Information

• NCBI GI #: 11177872
• NCBI GeneID: 29708
• NCBI Accession #: NP_068613.1
• NCBI GenBank Nucleotide #: NM_021841.1
• UniProt Accession #: P30191
• Molecular Weight: 57
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit alpha-6
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, alpha 6
• NCBI Official Symbol: Gabra6
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit alpha-6; GABA-A receptor alpha-6 subunit; GABA(A) receptor subunit alpha-6; gamma-aminobutyric acid A receptor, alpha 6; gamma-aminobutyric acid A receptor, alpha 6; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 6
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit alpha-6
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: Gabra6
• UniProt Synonym Gene Names: Gabra-6
• UniProt Entry Name: GBRA6_RAT

NCBI Description

GABAA receptor subunit; part of a cerebellar receptor subtype, selective for antagonist of alcohol-induced motor incoordination and ataxia [RGD, Feb 2006]

Uniprot Description

Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure: Binds UBQLN1

By similarity. Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue specificity: Only found in cerebellar granule cells.

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA6 sub-subfamily. [View classification]