Rabbit GABAA Receptor Polyclonal Antibody | anti-GABRA2 antibody

Anti-GABAA Receptor, alpha2-Subunit

Cat. Num. AAA502221

• Applications: Western Blot
• Purity: Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
• Synonyms: GABAA Receptor; Polyclonal Antibody; Anti-GABAA Receptor; alpha2-Subunit; GABA A Receptor a 2; anti-GABRA2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Specific for the ~51k alpha2-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha2-subunit knockout animals.
• Purity/Purification: Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 451

• Applicable Applications for anti-GABRA2 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen Species: Rat
• Immunogen Info: Fusion protein from the cytoplasmic loop of the alpha 2 subunit
• Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl-channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutictargets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseasesincluding epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
• Antigen: Fusion protein from the cytoplasmic loop of the alpha2-subunit of rat GABAA receptor.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot of mouse brain lysates from wild type (Control) and alpha2-knockout (alpha2-K/O) animals showing specific immunolabeling of the ~51k alpha2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha2-knockout animals.)

NCBI and Uniprot Product Information

• NCBI GI #: 120758
• NCBI GeneID: 289606
• UniProt Accession #: P23576
• Molecular Weight: ~51 kDa
• NCBI Official Full Name: Gamma-aminobutyric acid receptor subunit alpha-2
• NCBI Official Synonym Full Names: gamma-aminobutyric acid type A receptor alpha2 subunit
• NCBI Official Symbol: Gabra2
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit alpha-2
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit alpha-2
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: Gabra2
• UniProt Synonym Gene Names: Gabra-2
• UniProt Entry Name: GBRA2_RAT

NCBI Description

may act as subunits in GABA-A receptors with benzodiazepine II binding characteristics [RGD, Feb 2006]

Uniprot Description

GABRA2: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily.

Protein type: Membrane protein, integral; Transporter; Transporter, ion channel; Membrane protein, multi-pass

Cellular Component: axon; cell junction; cell soma; dendrite; integral to membrane; integral to synaptic vesicle membrane; plasma membrane; postsynaptic membrane; synapse

Molecular Function: benzodiazepine receptor activity; chloride channel activity; extracellular ligand-gated ion channel activity; GABA-A receptor activity

Biological Process: chloride transport; gamma-aminobutyric acid signaling pathway; neurotransmitter transport; regulation of neurotransmitter levels; synaptic transmission