Rabbit NMDA NR2A Subunit (Tyr1325) Polyclonal Antibody | anti-GRIN2A antibody

Anti-Phospho-Tyr1325 NMDA NR2A-Subunit

Cat. Num. AAA502160

• Gene Names: Grin2a; NR2A; GluN2A; NMDAR2A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: NMDA NR2A Subunit (Tyr1325); Polyclonal Antibody; Anti-Phospho-Tyr1325 NMDA NR2A-Subunit; anti-GRIN2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely blocked by blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 150 mM NaCl, 10mM HEPES, 100ug per ml BSA and 50% glycerol, pH 7.5. Adequate amount of material to conduct 10 mini western blots.
• Sequence Length: 1464

• Applicable Applications for anti-GRIN2A antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of rat NMDA NR2A.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 conjugated to KLH
• Quality Control: Western blots performed on each lot
• Immunogen Species: Rat
• Predicted Species Reactivity: Bovine, Canine, Hamster, Sheep. Predicted species reactivity is based on 100% homology with sequence used as antigen.
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissues. It is anticipated that the antibody will react with bovine and canine based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot of rat hippocampal lysate showing specific immuno labeling of the ~180 kDa NR2A subunit of the NMDAR phosphorylated at Tyr1325 in the first lane (-). Phospho specificity is shown a in the second lane (+) where immuno labeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).)

Related Product Information for anti-GRIN2A antibody

Affinity purified rabbit polyclonal antibody

References

• Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003)Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33. Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M, Nakanishi S (1993) Molecular characterization of the family of the N-methyl- D-aspartate receptor subunits. J Biol Chem 268:2836-2843. Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724. Snell LD, Nunley KR, Lickteig RL, Browning MD, Tabakoff B, Hoffman PL (1996) Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Mol Brain Res 40:71-78. Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358. King JE, Eugenin EA, Hazleton JE, Morgello S, Berman JW (2010) Mechanisms of HIV-tat-induced phosphorylation of N-methyl-D-aspartate receptor subunit 2A in human primary neurons: implications for neuroAIDS pathogenesis. Am J Pathol. 176(6):2819-30.
• Taniguchi S, Nakazawa T, Tanimura A, Kiyama Y, Tezuka T, Watabe AM, Katayama N, Yokoyama K, Inoue T, Izumi-Nakaseko H, Kakuta S, Sudo K, Iwakura Y, Umemori H, Inoue T, Murphy NP, Hashimoto K, Kano M, Manabe T, Yamamoto T. (2009) Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour. EMBO J. 28(23):3717-29.

NCBI and Uniprot Product Information

• NCBI GI #: 140971205
• NCBI GeneID: 24409
• NCBI Accession #: NP_036705.3
• NCBI GenBank Nucleotide #: NM_012573.3
• UniProt Accession #: Q00959; O08948; Q63728
• Molecular Weight: ~180 kDa
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2A
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• NCBI Official Symbol: Grin2a
• NCBI Official Synonym Symbols: NR2A; GluN2A; NMDAR2A
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; glutamate [NMDA] receptor subunit epsilon-1
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2A
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin2a
• UniProt Synonym Gene Names: GluN2A; NMDAR2A; NR2A
• UniProt Entry Name: NMDE1_RAT

NCBI Description

may play a role in synaptic transmission, learning and memory [RGD, Feb 2006]

Uniprot Description

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with AIP1. Found in a complex with GRIN1 and GRIN3B. Interacts with HIP1 and NETO1. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes

By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with LRFN2. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein Ref.8.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. [View classification]