Rabbit anti-Rat GABAA Receptor, b3 (Ser408/409) Polyclonal Antibody | anti-GABRB3 antibody

Anti-Phospho-Ser408/409 GABAA Receptor, beta3 subunit

Cat. Num. AAA502162

• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Synonyms: GABAA Receptor; b3 (Ser408/409); Polyclonal Antibody; Anti-Phospho-Ser408/409 GABAA Receptor; beta3 subunit; anti-GABRB3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~53k GABAA receptor beta3 subunit phosphorylated at Ser408/409. Immunolabeling of the GABAA band is completely blocked by the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots
• Sequence Length: 473

• Applicable Applications for anti-GABRB3 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the di-phospho-Ser408/409 of the rat GABAA receptor, beta3 subunit.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser408/409 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, mouse, human, non-human primate, Xenopus and zebrafish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. Phosphorylation of serine 408 and serine 409 within the beta3 subunit have been shown to be critical for the functional modulation of beta3 containing recombinant receptors (Brandon et al., 2000).
• Preparation and Storage: Store at -20 degree C; stable for at least one year.

Testing Data

Related Product Information for anti-GABRB3 antibody

Affinity purified rabbit polyclonal antibody

References

• Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.
• Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629.
• Brandon NJ, Delmas P, Kittler JT, McDonald BJ, Sieghart W, Brown DA, Smart TG, Moss SJ (2000) GABAA receptor phosphorylation and functional modulation in cortical neurons by a protein kinase C-dependent pathway. J Biol Chem 275, 38856-62
• Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645- 653

NCBI and Uniprot Product Information

• NCBI GI #: 8393390
• NCBI GeneID: 24922
• NCBI Accession #: NP_058761.1
• NCBI GenBank Nucleotide #: NM_017065.1
• UniProt Accession #: P63079; P15433
• Molecular Weight: 53
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit beta-3
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, beta 3
• NCBI Official Symbol: Gabrb3
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit beta-3; GABA(A) receptor subunit beta-3; Gamma-aminobutyric acid receptor beta 3; gamma-aminobutyric acid receptor, subunit beta 3; testis gamma-aminobutyric acid receptor subunit beta 3; gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit beta-3
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: Gabrb3
• UniProt Synonym Gene Names: Gabrb-3
• UniProt Entry Name: GBRB3_RAT

NCBI Description

beta 3 subunit, with alpha subunits forms active GABA-A receptors [RGD, Feb 2006]

Uniprot Description

Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. [View classification]