Rabbit anti-Rat DOPA Decarboxylase, Bovine Polyclonal Antibody | anti-DDC antibody

Anti-DOPA Decarboxylase

Cat. Num. AAA502020

• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification using an AminoLink Plus column matrix to which purified, recombinant bovine DDC was coupled.)
• Synonyms: DOPA Decarboxylase; Bovine; Polyclonal Antibody; Anti-DOPA Decarboxylase; anti-DDC antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~55k DDC protein.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification using an AminoLink Plus column matrix to which purified, recombinant bovine DDC was coupled.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 487

• Applicable Applications for anti-DDC antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: SDS denatured, recombinant bovine aromatic DOPA decarboxylase (DDC) expressed in E. coli and purified from inclusion bodies.
• Immunogen Information: SDS denatured, recombinant bovine aromatic DOPA decarboxylase expressed in E. coli and purified from inclusion bodies
• Immunogen Species: Bovine
• Reactivity Assumed Based on 100% Sequence Homology: Bovine
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat tissues.
• Biological Significance: DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-DDC antibody

Affinity purified rabbit polyclonal antibody

References

• Berry MD, Juorio AV, Li XM, Boulton AA (1996) Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem Res 21:1075-1087.
• Haycock JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585.
• Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
• Waymire JC, Haycock JW (2002) Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J Neurochem 81:589-593.
• Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.

NCBI and Uniprot Product Information

• NCBI GI #: 27806901
• NCBI GeneID: 280762
• NCBI Accession #: NP_776332.1
• NCBI GenBank Nucleotide #: NM_173907.2
• UniProt Accession #: P27718; Q0V8S3
• Molecular Weight: 55
• NCBI Official Full Name: aromatic-L-amino-acid decarboxylase
• NCBI Official Synonym Full Names: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• NCBI Official Symbol: DDC
• NCBI Protein Information: aromatic-L-amino-acid decarboxylase; AADC; aromatic L-amino acid decarboxylase
• UniProt Protein Name: Aromatic-L-amino-acid decarboxylase
• Protein Family: Aromatic-L-amino-acid decarboxylase
• UniProt Gene Name: DDC
• UniProt Synonym Gene Names: AADC; DDC
• UniProt Entry Name: DDC_BOVIN

Uniprot Description

Function: Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.

Catalytic activity: L-dopa = dopamine + CO2.5-hydroxy-L-tryptophan = 5-hydroxytryptamine + CO2.

Cofactor: Pyridoxal phosphate.

Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.

Subunit structure: Homodimer.

Sequence similarities: Belongs to the group II decarboxylase family.