Rabbit anti-Rat GABA Transporter (GAT) 1 Polyclonal Antibody | anti-SLC6A1 antibody

Anti-GABA Transporter (GAT) 1

Cat. Num. AAA502038

• Gene Names: Slc6a1; Gat1; Gabt1
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification.)
• Synonyms: GABA Transporter (GAT) 1; Polyclonal Antibody; Anti-GABA Transporter (GAT) 1; anti-SLC6A1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~67k GAT-1 protein. Immunolabeling is blocked by the peptide used as antigen.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 599

• Applicable Applications for anti-SLC6A1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000; IHC: 1:100-1:200
• Antigen: Peptide corresponding to amino acid residues from the C-terminal region of rat GAT-1.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Mouse
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with mouse tissue based on the fact that this species has 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-SLC6A1 antibody

Affinity purified rabbit polyclonal antibody

References

• Minelli A, Brecha NC, Karschin C, DeBiasi S, Conti F (1995) GAT-1, a high-affinity GABA plasma membrana transporter, is localizad to neurons and astroglia int he cerebral cortex. J. Neurosci. 15(11):7734-7746.
• Zhao WJ, Ma YH, Fei J, Mei ZT, Guo LH (2003) Increase in drug-induced seizure susceptibility of transgenic mice overexpressing GABA transporter 1. Acta Pharmacol. Sin. 24(10):991-5.

NCBI and Uniprot Product Information

• NCBI GI #: 13242269
• NCBI GeneID: 79212
• NCBI Accession #: NP_077347.1
• NCBI GenBank Nucleotide #: NM_024371.2
• UniProt Accession #: P23978
• Molecular Weight: 67
• NCBI Official Full Name: sodium- and chloride-dependent GABA transporter 1
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 1
• NCBI Official Symbol: Slc6a1
• NCBI Official Synonym Symbols: Gat1; Gabt1
• NCBI Protein Information: sodium- and chloride-dependent GABA transporter 1; GAT-1; GABA transporter protein; solute carrier family 6 member 1; solute carrier family 6 (neurotransmitter transporter, GABA), member 1
• UniProt Protein Name: Sodium- and chloride-dependent GABA transporter 1
• Protein Family: Sodium- and chloride-dependent GABA transporter
• UniProt Gene Name: Slc6a1
• UniProt Synonym Gene Names: Gabt1; Gat-1; Gat1; GAT-1
• UniProt Entry Name: SC6A1_RAT

NCBI Description

transporter for the neurotransmitter gamma-aminobutyric acid (GABA) [RGD, Feb 2006]

Uniprot Description

Function: Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

Subunit structure: Interacts with MPP5. Ref.3

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Membrane; Multi-pass membrane protein. Note: Localized at the presynaptic terminals of interneurons

By similarity. Localized at the plasma membrane and in a subset of intracellular vesicles. Ref.3

Domain: The PDZ domain-binding motif is involved in the interaction with MPP5

By similarity.

Miscellaneous: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Sequence similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. [View classification]