Rabbit DOPA Decarboxylase, Human Polyclonal Antibody | anti-DDC antibody

Anti-DOPA Decarboxylase

Cat. Num. AAA502021

• Gene Names: DDC; AADC
• Reactivity: Bovine, canine, guinea pig, human, rabbit, rat and sheep tissues
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification using an AminoLink Plus column matrix to which purified, recombinant bovine DDC was coupled.)
• Synonyms: DOPA Decarboxylase; Human; Polyclonal Antibody; Anti-DOPA Decarboxylase; anti-DDC antibody

• Host: Rabbit
• Reactivity: Bovine, canine, guinea pig, human, rabbit, rat and sheep tissues
• Clonality: Polyclonal
• Specificity: Specific for the ~55k DDC protein
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification using an AminoLink Plus column matrix to which purified, recombinant bovine DDC was coupled.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 480

• Applicable Applications for anti-DDC antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Peptide from N-terminus region of human DOPA decarboxylase (DDC), conjugated to keyhole limpet hemocyanin (KLH).
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
• Immunogen Species: Human
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in bovine, canine, guinea pig, human, rabbit, rat and sheep tissues.
• Biological Significance: DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-DDC antibody

Affinity purified rabbit polyclonal antibody

References

• Berry MD, Juorio AV, Li XM, Boulton AA (1996) Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem Res 21:1075-1087.
• Haycock JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585.
• Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
• Waymire JC, Haycock JW (2002) Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J Neurochem 81:589-593.
• Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.

NCBI and Uniprot Product Information

• NCBI GI #: 4503281
• NCBI GeneID: 1644
• NCBI Accession #: NP_000781.1
• NCBI GenBank Nucleotide #: NM_000790.3
• UniProt Accession #: P20711; Q16723; Q5W5T9; Q75MJ6; C9IYA0; E7ER62; E7EU95
• Molecular Weight: 55
• NCBI Official Full Name: aromatic-L-amino-acid decarboxylase isoform 1
• NCBI Official Synonym Full Names: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• NCBI Official Symbol: DDC
• NCBI Official Synonym Symbols: AADC
• NCBI Protein Information: aromatic-L-amino-acid decarboxylase
• UniProt Protein Name: Aromatic-L-amino-acid decarboxylase
• Protein Family: Aromatic-L-amino-acid decarboxylase
• UniProt Gene Name: DDC
• UniProt Synonym Gene Names: AADC; AADC; DDC
• UniProt Entry Name: DDC_HUMAN

NCBI Description

The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Function: Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.

Catalytic activity: L-dopa = dopamine + CO2.5-hydroxy-L-tryptophan = 5-hydroxytryptamine + CO2.

Cofactor: Pyridoxal phosphate. Ref.10

Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.

Subunit structure: Homodimer. Ref.10

Involvement in disease: Aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]: An inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.3 Ref.11 Ref.14 Ref.15

Sequence similarities: Belongs to the group II decarboxylase family.