Immunofluorescence (IF)
(Acf1 pAb tested by immunofluorescence. Drosophila polytene chromosomes stained with Acf1 pAb (1:1,000 dilution).)
Rabbit anti-Drosophila Acf1 Polyclonal Antibody | anti-Acf1 antibody
Acf1 antibody (pAb)
Reactivity
Drosophila
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity Purified
Synonyms
Acf1; Polyclonal Antibody; Acf1 antibody (pAb); Acf; ACF; Acf-1; ACF/CHRAC; ACF1; CAF; CG1966; CHRAC; CHRAC-175; dACF; dCHRAC; Dmel\CG1966; p170/p185; anti-Acf1 antibody
Host
Rabbit
Reactivity
Drosophila
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.1% sodium azide, 0.2mg/ml BSA and 35% glycerol. Sodium azide is highly toxic.
Applicable Applications for anti-Acf1 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications Validated:
ICC/IF: 1:500-1:1,000 dilution
WB: 1:500-1:1,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
ICC/IF: 1:500-1:1,000 dilution
WB: 1:500-1:1,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Immunogen
This Acf1 antibody was raised against recombinant C-terminal fragment of Acf1 from Drosophila melanogaster.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Immunofluorescence (IF)
(Acf1 pAb tested by immunofluorescence. Drosophila polytene chromosomes stained with Acf1 pAb (1:1,000 dilution).)
Immunofluorescence (IF)
(Acf1 pAb tested by immunofluorescence. Drosophila polytene chromosomes stained with Acf1 pAb (1:1,000 dilution).)
Western Blot (WB)
(Acf1 pAb tested by Western blot. Drosophila Schneider L2 cells nuclear extract (10 ug) probed with Acf1 pAb (1:500 dilution).)
Western Blot (WB)
(Acf1 pAb tested by Western blot. Drosophila Schneider L2 cells nuclear extract (10 ug) probed with Acf1 pAb (1:500 dilution).)
Related Product Information for anti-Acf1 antibody
Short Description: Our Acf1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Drosophila samples.
Background: Drosophila Acf1 is a component of ACF, the ATP-utilizing chromatin assembly and remodeling factor protein complex. ACF catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro. The catalytic subunit of ACF is the ISWI ATP-dependant chromatin remodeling protein. ACF is related to CHRAC (Chromatin Accessibility Complex), which also contains Acf1 and ISWI. ACF is involved in the establishment and possibly maintenance of silenced heterochromatic domains in the genome, both at pericentric heterochromatin and at genes repressed by the Polycomb complex.
Background: Drosophila Acf1 is a component of ACF, the ATP-utilizing chromatin assembly and remodeling factor protein complex. ACF catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro. The catalytic subunit of ACF is the ISWI ATP-dependant chromatin remodeling protein. ACF is related to CHRAC (Chromatin Accessibility Complex), which also contains Acf1 and ISWI. ACF is involved in the establishment and possibly maintenance of silenced heterochromatic domains in the genome, both at pericentric heterochromatin and at genes repressed by the Polycomb complex.
Product Categories/Family for anti-Acf1 antibody
NCBI and Uniprot Product Information
Molecular Weight
185kDa
Similar Products
Product Notes
The Acf1 (Catalog #AAA388553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acf1 antibody (pAb) reacts with Drosophila and may cross-react with other species as described in the data sheet. AAA Biotech's Acf1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Applications Validated: ICC/IF: 1:500-1:1,000 dilution WB: 1:500-1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the Acf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Acf1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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