Immunofluorescence (IF)
(SMARCA5 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCA5 antibody at a 1:2,000 dilution.)
Mouse anti-Human, Mouse SMARCA5 Monoclonal Antibody | anti-SMARCA5 antibody
SMARCA5 antibody (mAb)
Gene Names
SMARCA5; ISWI; SNF2H; hISWI; hSNF2H; WCRF135
Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Ascites
Synonyms
SMARCA5; Monoclonal Antibody; SMARCA5 antibody (mAb); SMARCA5 SWI/SNF; ISWI; hISWI; hSNF2H; WCRF135; anti-SMARCA5 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Ascites
Form/Format
Ascites containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-SMARCA5 antibody
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
ICC/IF: 1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
ICC/IF: 1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
Immunogen
This antibody was raised against purified human RSF complex which was used to immunize mice.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Immunofluorescence (IF)
(SMARCA5 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCA5 antibody at a 1:2,000 dilution.)
Immunofluorescence (IF)
(SMARCA5 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCA5 antibody at a 1:2,000 dilution.)
Western Blot (WB)
(SMARCA5 antibody (mAb) tested by Western blot. HeLa acid extract (10 ug) was probed with SMARCA5 mAb (1:2,000 dilution).)
Western Blot (WB)
(SMARCA5 antibody (mAb) tested by Western blot. HeLa acid extract (10 ug) was probed with SMARCA5 mAb (1:2,000 dilution).)
Related Product Information for anti-SMARCA5 antibody
Short Description: Our SMARCA5 antibody (mAb) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Human and Mouse samples.
Background: SMARCA5 is a human homolog of the budding yeast Snf2 protein. SMARCA5 is the catalytic subunit of several chromatin remodeling complexes, such as CHRAC, RSF, ACF, NuRD and NoRC. SMARCA5 is a member of the SWI/SNF superfamily of proteins and members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA5 is a component of the chromatin remodeling and spacing factor RSF (Rsf1), a facilitator of the transcription of class II genes by RNA pol II. It is similar in sequence to the Drosophila ISWI chromatin remodeling protein. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
Background: SMARCA5 is a human homolog of the budding yeast Snf2 protein. SMARCA5 is the catalytic subunit of several chromatin remodeling complexes, such as CHRAC, RSF, ACF, NuRD and NoRC. SMARCA5 is a member of the SWI/SNF superfamily of proteins and members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA5 is a component of the chromatin remodeling and spacing factor RSF (Rsf1), a facilitator of the transcription of class II genes by RNA pol II. It is similar in sequence to the Drosophila ISWI chromatin remodeling protein. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
Product Categories/Family for anti-SMARCA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
122kDa
NCBI Official Full Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
NCBI Official Symbol
SMARCA5
NCBI Official Synonym Symbols
ISWI; SNF2H; hISWI; hSNF2H; WCRF135
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
UniProt Gene Name
SMARCA5
UniProt Synonym Gene Names
SNF2H; WCRF135; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; hSNF2H
UniProt Entry Name
SMCA5_HUMAN
NCBI Description
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]
Research Articles on SMARCA5
Similar Products
Product Notes
The SMARCA5 smarca5 (Catalog #AAA388484) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SMARCA5 antibody (mAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA5 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: ICC/IF: 1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. Researchers should empirically determine the suitability of the SMARCA5 smarca5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCA5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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