Rabbit anti-Human Elk-1 Polyclonal Antibody | anti-Elk-1 antibody

Elk-1 antibody (pAb)

Cat. Num. AAA388849

• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Affinity Purified
• Synonyms: Elk-1; Polyclonal Antibody; Elk-1 antibody (pAb); anti-Elk-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-Elk-1 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications Validated:; IP: 10ul per IP; WB: 1:500-1:1,000 dilution; The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
• Immunogen: This Elk-1 antibody was raised against a peptide within the C-terminal region of human Elk-1.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(Elk-1 antibody (pAb) tested by Immunoprecipitation. 10 ul of Elk-1 antibody was used to immunoprecipitate Elk-1 from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Elk-1 antibody at a dilution of 1:500.)

Western Blot (WB)

(Elk-1 antibody (pAb) tested by Western blot. Whole cell extract of HeLa cells (40 ug) probed with Elk-1 antibody (1:500).)

Related Product Information for anti-Elk-1 antibody

Short Description: Our Elk-1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.

Background: Elk-1 (ETS-like gene 1) is a member of the ETS family of transcription factors and stimulates transcription. It binds to purine-rich DNA sequences, and can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.

Product Categories/Family for anti-Elk-1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 166362730
• NCBI GeneID: 2002
• NCBI Accession #: NP_005220; AB_2793587
• NCBI GenBank Nucleotide #: NM_005229
• UniProt Accession #: P19419
• Molecular Weight: 45kDa
• NCBI Official Full Name: ETS domain-containing protein Elk-1 isoform a
• NCBI Official Synonym Full Names: ETS transcription factor ELK1
• NCBI Official Symbol: ELK1
• NCBI Protein Information: ETS domain-containing protein Elk-1
• UniProt Protein Name: ETS domain-containing protein Elk-1
• UniProt Gene Name: ELK1
• UniProt Entry Name: ELK1_HUMAN

NCBI Description

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Uniprot Description

ELK1: a transcription factor of the Ets family and the ternary complex factor (TCF) subfamily. Forms a ternary complex by binding to the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. Mediates gene activity in response to serum and growth factors. Phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites is critical for transcriptional activation by Elk-1. Two alternatively-spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: nucleoplasm; mitochondrion; cell soma; dendrite; nerve terminal; nucleus

Molecular Function: protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to light stimulus; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway