Rabbit CENP-A phospho Ser18 Polyclonal Antibody | anti-CENP-A antibody

CENP-A phospho Ser18 antibody (pAb)

Cat. Num. AAA388916

• Gene Names: CENPA; CenH3; CENP-A
• Reactivity: Human, Wide Range Predicted
• Applications: Dot Blot, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Affinity Purified
• Synonyms: CENP-A phospho Ser18; Polyclonal Antibody; CENP-A phospho Ser18 antibody (pAb); CenH3; CENPA; anti-CENP-A antibody

• Host: Rabbit
• Reactivity: Human, Wide Range Predicted
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-CENP-A antibody: Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications Validated:; ICC/IF: 1:100 dilution; WB: 1:100-1:500 dilution; The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
• Conjugation: Phosphorylated
• Immunogen: This antibody was raised against a synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A.
• Abs Residue: Ser
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Immunofluorescence (IF)

(CENP-A phospho Ser18 antibody (pAb) tested by immunofluorescence. Detection of CENP-A phospho Ser18 by immunofluorescence. HeLa cells were stained with CENP-A phospho Ser18 antibody at a 1:100 dilution. Green: CENP-A phospho Ser18 antibody (pAb) Blue: DAPI)

Western Blot (WB)

(CENP-A phospho Ser18 antibody (pAb) tested by Western blot. Top: Recombinant CENP-A (20 ng) was probed with CENP-A phospho Ser18 antibody (1:100 dilution) (A) or with anti-centromere antibody (ACA) (B). CENP-A phospho Ser18 antibody does not detect recombinant CENP-A. Bottom: HeLa nuclear extract (20 ug) was probed with CENP-A phospho Ser18 antibody (1:250 dilution). Lane 1: CENP-A phospho-Ser18 antibody. Lane 2: anti-centromere antibody (ACA).)

Dot Blot (DB)

(CENP-A phospho Ser18 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of CENP-A phospho Ser18 antibody. Peptides corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 1:750. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: CENP-A phospho Ser 16 and phospho Ser18. Lane 2: CENP-A unmodified. Lane 3: CENP-A phospho Ser 16. Lane 4: CENP-A phospho Ser 18.)

Related Product Information for anti-CENP-A antibody

Short Description: Our CENP-A phospho Ser18 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Background: CENP-A (Centromere Protein A) is a Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. Recently, phosphorylation of CENP-A on Ser16 and Ser18 was reported and found to be important for chromosome segregation during mitosis.

Product Categories/Family for anti-CENP-A antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502775
• NCBI GeneID: 1058
• NCBI Accession #: NP_001800; AB_2793655
• NCBI GenBank Nucleotide #: NM_001809
• UniProt Accession #: P49450
• Molecular Weight: 16kDa
• NCBI Official Full Name: histone H3-like centromeric protein A isoform a
• NCBI Official Synonym Full Names: centromere protein A
• NCBI Official Symbol: CENPA
• NCBI Official Synonym Symbols: CenH3; CENP-A
• NCBI Protein Information: histone H3-like centromeric protein A
• UniProt Protein Name: Histone H3-like centromeric protein A
• UniProt Gene Name: CENPA
• UniProt Synonym Gene Names: CENP-A
• UniProt Entry Name: CENPA_HUMAN

NCBI Description

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]

Uniprot Description

CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; inner kinetochore of condensed chromosome; nucleosome; cytosol; nucleus; chromosome, pericentric region; condensed nuclear chromosome, pericentric region

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; chromatin binding

Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; viral reproduction; establishment of mitotic spindle orientation; mitotic cell cycle