Western Blot (WB)
(HIRA mAb (Clone WC119.2H11) tested by Western blot. 20 ug of cell extract from HIRA-transfected HEK293T cells was probed with antibody at 2 ug/ml.)
Mouse anti-Human, Mouse HIRA Monoclonal Antibody | anti-HIRA antibody
HIRA antibody (mAb)(Clone WC119.2H11)
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Protein G Chromatography
Synonyms
HIRA; Monoclonal Antibody; HIRA antibody (mAb)(Clone WC119.2H11); TUP1; DGCR1; TUPLE1; anti-HIRA antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
WC119.2H11
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-HIRA antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results, we recommend the addition of 0.05% Tween-20 to all blocking solutions to reduce background in Western blot.
WB: 1-2ug/ml dilution
For optimal results, we recommend the addition of 0.05% Tween-20 to all blocking solutions to reduce background in Western blot.
Immunogen
This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(HIRA mAb (Clone WC119.2H11) tested by Western blot. 20 ug of cell extract from HIRA-transfected HEK293T cells was probed with antibody at 2 ug/ml.)
Western Blot (WB)
(HIRA mAb (Clone WC119.2H11) tested by Western blot. 20 ug of cell extract from HIRA-transfected HEK293T cells was probed with antibody at 2 ug/ml.)
Related Product Information for anti-HIRA antibody
Short Description: Our HIRA antibody (mAb)(Clone WC119.2H11) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples.
Background: HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1a is involved in the formation of the senescence-associated heterochromatin foci.
Background: HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1a is involved in the formation of the senescence-associated heterochromatin foci.
Product Categories/Family for anti-HIRA antibody
NCBI and Uniprot Product Information
Molecular Weight
120kDa
Protein Family
Similar Products
Product Notes
The HIRA (Catalog #AAA388476) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HIRA antibody (mAb)(Clone WC119.2H11) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HIRA can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, we recommend the addition of 0.05% Tween-20 to all blocking solutions to reduce background in Western blot. Researchers should empirically determine the suitability of the HIRA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIRA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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